Indocyanine green (Cardiogreen) is a low toxicic fluorescent agent, is a tricarbocyanine dye,has been widely used in medical diagnostics, such as determining cardiac output, hepatic function, and liver blood flow, and for ophthalmic angiography.

Cardiogreen(ICG-PDT) at concentrations 1000μg/mL, induced the significant expression of BAX in HGF cells; however, the laser irradiation as well as ICG showed no significant effects on the expression of these genes. Treatment with ICG alone, laser irradiation and ICG-PDT caused no observable BCL-2 gene expression changes between the tested and control groups.

Indocyanine green is used to detect the distribution of oral polysaccharide nanoemulsions
Operation steps:
a. Dissolve indocyanine green in polysaccharide aqueous solution and then add to nanoemulsion to form a complex (the final concentration of indocyanine green in both solutions is 0.05 mg/mL);
b. After oral administration of the complex, mice were fasted for 4 hours and then placed in a real-time imaging device to observe the distribution of fluorescence in vivo.
Cell experiment operation steps:
a. Add indocyanine green at a final concentration of 1mg/mL to serum-free hepatocyte culture medium and culture at 37℃ for 1 hour;
b. Remove serum-free culture medium and wash cells three times with PBS;
c. Examine and image cells under a microscope.