Ganoderic acid S, a positional isomer of ganoderic acids, can be isolated from the fermented mycelia of Ganoderma lucidum. It can induce apoptosis in HeLa cells through the mitochondria pathway [1].

Ganoderic acid S at concentrations of 0, 39.1, and 97.7 μM over 12 hours causes cell cycle arrest in the S phase in HeLa cells. It induces a dose-dependent collapse of mitochondrial membrane potential and promotes significant release of cytochrome c from mitochondria into the cytosol of HeLa cells. [1] Cell Cycle Analysis [1] Cell Line: HeLa cells Concentration: 0, 39.1, 97.7 μM Incubation Time: 12 h Result: Caused cell cycle arrest in the S phase.