G0-C14 is a cationic lipid-like molecule commonly used for synthesizing nanoparticles and vaccine delivery.

G0-C14 has a highly efficient encapsulation ability for mRNA and pDNA, and the encapsulation efficiency can reach more than 95%. [1]
The nanoparticles were prepared as follows:[1]
1. PolyHCPT and DSPE-PEG3K were dissolved in DMF and prepared into a homogeneous solution with a concentration of 5 mg/mL.
2. a mixture of siRNA (0.1 nmol/µL in water) and G0-C14 (5 mg/mL, dissolved in DMF) was prepared and mixed according to different N/P molar ratios. Subsequently, this mixture is combined with PolyHCPT and DSPE-PEG3K solutions.
3. The above mixture was added dropwise to 5 mL of deionized water at 1000 rpm with vigorous stirring.
4. The resulting NPs dispersion was transferred to an ultrafiltration unit.
5. Centrifuge at 2800 rpm for 8 minutes at room temperature to remove organic solvents and free compounds.