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Fluorescein diacetate

(Synonyms: Di-O-acetylfluorescein, 3,6-Diacetoxyfluoran) Copy Product Info

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Synonyms: Di-O-acetylfluorescein, 3,6-Diacetoxyfluoran

Catalog No. T19323 Copy Product Info

Purity: 99.79%

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Fluorescein diacetate (Di-O-acetylfluorescein) is a cell permeable esterase-substrate for hGSTP1-1.

Cas No. 596-09-8

Pack Size	Price	USA Stock	Global Stock	Quantity
500 mg	$42	In Stock	In Stock
1 mL x 10 mM (in DMSO)	$46	In Stock	In Stock

For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)

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For research use only—not for human use. No sales to individuals. Use as intended only.

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Purity:99.79%

Color:White to Yellow

Resource Download

COA HNMR LCMS

Product Introduction

Bioactivity

Description	Fluorescein diacetate (Di-O-acetylfluorescein) is a cell permeable esterase-substrate for hGSTP1-1.
In vitro	Fluorescein diacetate (FDA) serves as a fluorogenic substrate in assessing the efficacy of inhibitors against the enzyme GSTP1-1 in vitro. Notably, Ethacrynic acid (EA) and NBDHEX are effective in inhibiting the enzyme-activated fluorescence of FDA, demonstrating concentration-dependent inhibition with IC50 values of 3.3±0.5 μM and 0.61±0.04 μM, respectively. FDA, an acetylated variant of fluorescein, is activated fluorescently through the esterolytic action of the human Pi-class glutathione S-transferase (hGSTP1), marking it specific among various cytosolic GSTs. This fluorogenic activity allows for the usage of FDA in vital staining. Particularly, when MCF7 cells overexpress hGSTP1, an observable fluorescence activation due to GST inhibitors is noted, an effect not seen in cells overexpressing hGSTA1 or hGSTM1. The enzyme-dependent fluorescence activation is further corroborated by incubating FDA with recombinant hGSTP1-1 and GSH, showing significant fluorescence only in the presence of both, indicating the necessity of hGSTP1-1 activity for such activation. This specificity is underscored by the established linear relation between fluorescence increase rate and hGSTP1-1 concentration, determining the enzyme's specific activity for FDA to be 79±15 nmol/min/mg protein.
Synonyms	Di-O-acetylfluorescein, 3,6-Diacetoxyfluoran

Chemical Properties

Molecular Weight	416.38
Formula	C₂₄H₁₆O₇
Cas No.	596-09-8
Smiles	CC(=O)Oc1ccc2c(Oc3cc(OC(C)=O)ccc3C22OC(=O)c3ccccc23)c1
Relative Density.	1.2738 g/cm3 (Estimated)

Storage & Solubility Information

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 73.57 mg/mL (176.69 mM), Sonication is recommended.

In Vivo Formulation

10% DMSO+40% PEG300+5% Tween 80+45% Saline: 7.36 mg/mL (17.68 mM), Solution.

10% DMSO+90% Saline: < 7.36 mg/mL (17.68 mM), Lower concentrations may be soluble, but exact solubility limit is unknown.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	2.4017 mL	12.0083 mL	24.0165 mL	120.0826 mL
5 mM	0.4803 mL	2.4017 mL	4.8033 mL	24.0165 mL
10 mM	0.2402 mL	1.2008 mL	2.4017 mL	12.0083 mL
20 mM	0.1201 mL	0.6004 mL	1.2008 mL	6.0041 mL
50 mM	0.0480 mL	0.2402 mL	0.4803 mL	2.4017 mL
100 mM	0.0240 mL	0.1201 mL	0.2402 mL	1.2008 mL

Note : The dilution table applies only to solid products. For liquid products, please calculate the stock solution based on the stated concentration and/or density.

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 µL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 µL Tween 80 and mix well until fully clarified.

3) Add 450 µL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

Keywords

InhibitorinhibitFluorescein diacetateFluorescein

Related Tags: Fluorescein diacetate chemical structure | Fluorescein diacetate in vitro | Fluorescein diacetate formula | Fluorescein diacetate molecular weight