dASK1 is a selective CRBN-based PROTAC degrader targeting apoptosis signal-regulating kinase 1 (ASK1). It forms a stable ternary complex with ASK1, facilitating its rapid and sustained degradation via the ubiquitin-proteasome pathway. dASK1 exhibits strong ASK1 degradation capabilities and is applicable for hepatitis research.

dASK1 (Compound 35) induces ASK1 degradation in HepG2 and HEK293A cells at concentrations ranging from 1 to 5000 nM over 4 to 12 hours. In HepG2 cells, dASK1 shows no detectable toxicity at concentrations between 1 nM and 10 μM over 24 hours. The degradation of ASK1 by dASK1 (10-200 nM for 8 hours) in HepG2 cells is dependent on the proteasome.