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Bovine beta-1,4-galactosyltransferase 1 (Y289L) (Bovin B4GALT1 (Y289L)) is a mutated galactosyltransferase derived from cattle with a mutation at the Y289L gene locus. This enzyme labels O-GlcNAcylated proteins with an N-azidoacetylgalactosamine (GalNAz) group. This labeling technique enables specific, unbiased, and global tagging of O-GlcNAcylated proteins. After labeling, the attached azide group can react with various alkyne-modified chemical probes, facilitating a range of downstream analyses.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 10 mg | Inquiry | Inquiry | Inquiry | |
| 50 mg | Inquiry | Inquiry | Inquiry |
| Description | Bovine beta-1,4-galactosyltransferase 1 (Y289L) (Bovin B4GALT1 (Y289L)) is a mutated galactosyltransferase derived from cattle with a mutation at the Y289L gene locus. This enzyme labels O-GlcNAcylated proteins with an N-azidoacetylgalactosamine (GalNAz) group. This labeling technique enables specific, unbiased, and global tagging of O-GlcNAcylated proteins. After labeling, the attached azide group can react with various alkyne-modified chemical probes, facilitating a range of downstream analyses. |
| Synonyms | Bovin B4GALT1 (Y289L) |
| Storage | Powder: -20°C for 3 years | In solvent: -80°C for 1 year |
Dissolve 2 mg of the compound in 100 μL DMSO
to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.
1) Add 100 μL of the DMSO
stock solution to 400 μL PEG300
and mix thoroughly until the solution becomes clear.
2) Add 50 μL Tween 80 and mix well until fully clarified.
3) Add 450 μL Saline,PBS or ddH2O
and mix thoroughly until a homogeneous solution is obtained.
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