BBD (NSC-240867) (Benzylamino-NBD) is a biochemical and chromogenic reagent.

Instructions
1. Color reaction preparation:
Dissolve BBD (NSC240867) in an appropriate solvent or buffer (such as PBS or DMSO) to prepare a colorimetric solution of the desired concentration, typically in the range of 10 µM - 1 mM.
2. Experimental setup:
1) Prepare the sample or biological mixture (e.g., enzyme solution, cell culture supernatant, or other biological specimen).
2) Add the BBD solution to the sample, ensuring that it is mixed evenly.
3. Incubation and observation:
1) Incubate the mixture for a set time, which may vary depending on the reaction kinetics (usually 15 minutes to 1 hour).
2) Observe the color change, which will indicate the presence and concentration of the target analyte or enzyme activity.
3) For quantitative measurements, use a spectrophotometer to detect absorbance at the appropriate wavelength, typically in the visible spectrum.
4. Data interpretation: The extent of the color change can be directly related to the concentration of the analyte or enzyme activity in the sample. Convert absorbance readings to quantitative results using a standard curve or calibration data.