BAMEA-O16B

Name: BAMEA-O16B
Brand: TargetMol
SKU: T40153
Rating: 4.5 (1 reviews)

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Catalog No. T40153Cas No. 2490668-30-7

Alias BAMEA-O16B

BAMEAO16B is a lipid nanoparticle that incorporates disulfide bonds for efficient delivery of Cas9 mRNA and sgRNA into cells. In the intracellular environment, BAMEAO16B responds to reduction by releasing RNA, facilitating genome editing. This compound is valuable for gene editing research purposes.

BAMEA-O16B

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Catalog No. T40153Alias BAMEA-O16BCas No. 2490668-30-7

Pack Size	Price	USA Warehouse	Global Warehouse
5 mg	$258	35 days	35 days
10 mg	$485	35 days	35 days
25 mg	$1,150	35 days	35 days
50 mg	$1,980	35 days	35 days

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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

With extensive experience in compound synthesis, we can provide rapid custom synthesis services for this product according to your research needs.All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.

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Product Introduction

Bioactivity

Description	BAMEAO16B is a lipid nanoparticle that incorporates disulfide bonds for efficient delivery of Cas9 mRNA and sgRNA into cells. In the intracellular environment, BAMEAO16B responds to reduction by releasing RNA, facilitating genome editing. This compound is valuable for gene editing research purposes.
In vitro	Treatment with BAMEA-O16B/Cas9 mRNA/sgHPV18 significantly inhibits the growth of HeLa cells, showcasing superior effectiveness over the application of scrambled sgRNA and Cas9 mRNA delivery. BAMEA-O16B demonstrates notable efficiencies in RNA delivery, mRNA encapsulation, and GFP knockout, indicating its effectiveness in directing Cas9 mRNA to regulate endogenous gene expression accurately. Additionally, cells treated with BAMEA-O16B/RNA exhibit enhanced endosome escape, outperforming those treated with BAMEA-O16/RNA. Furthermore, nanoparticles composed of BAMEA-O16B/RFP mRNA achieve efficient RFP expression in HeLa cells, underscoring the compound’s proficiency in genetic manipulation and expression[1].
In vivo	The BAMEA-O16B/Cas9 mRNA/sgRNA (I.v.) nanoparticle significantly reduces the serum proprotein convertase subtilisin/kexin type 9 (PCSK9) levels in mice to 20% of those observed in untreated counterparts. Similarly, administration of BAMEA-O16B/Cas9 mRNA/sgPCSK9 nanoparticles lowers the PCSK9 concentration in mouse serum to 20% compared to levels following DPBS injection or treatment with BAMEA-O16B/Cas9 mRNA/scramblesgRNA nanoparticles[1].
Synonyms	BAMEA-O16B

Chemical Properties

Molecular Weight	1114.88
Formula	C₅₆H₁₁₁N₃O₆S₆
Cas No.	2490668-30-7
Smiles	CCCCCCCCCCCCSSCCOC(=O)CCNCCN(C)CCN(CCC(=O)OCCSSCCCCCCCCCCCC)CCC(=O)OCCSSCCCCCCCCCCCC

Storage & Solubility Information

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc