Anti-inflammatory agent 64 (compound 4b) demonstrates antioxidant and anti-inflammatory properties, effectively inhibiting IL-6 and TNF-α secretion and reducing paw edema [1] in both in vitro and in vivo studies.

Anti-inflammatory agent 64, at a concentration of 2 μM with 4 hours of incubation, markedly protects and promotes cell proliferation in RAW264.7 cells pre-stimulated with H2O2 (0.4 mM) [1].

Anti-inflammatory agent 64, when administered as a single dose ranging from 1-30 mg/kg via intraperitoneal injection or oral gavage, demonstrates anti-inflammatory and antioxidant activity in a mouse model of carrageenan (Cg)-induced paw edema [1].