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Amine-PEG3-Biotin

Name: Amine-PEG3-Biotin
Brand: TargetMol
SKU: T14220
Price: 29 USD
Availability: InStock
Rating: 5 (10 reviews)

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Catalog No. T14220Cas No. 359860-27-8

Amine-PEG3-Biotin can be used as a signal amplification label.

Amine-PEG3-Biotin

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🥰Excellent

Purity: 99.76%

Catalog No. T14220Cas No. 359860-27-8

Amine-PEG3-Biotin can be used as a signal amplification label.

Pack Size	Price	USA Warehouse	Global Warehouse	Quantity
25 mg	$29	In Stock	In Stock
50 mg	$49	In Stock	In Stock

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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.

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Batch Information

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Purity:99.76%

Appearance:Viscous

Color:Yellow

Resource Download

COA HNMR LCMS

Product Introduction

Bioactivity

Description

Amine-PEG3-Biotin can be used as a signal amplification label.

Cell Research

1. Binding to biological molecules
1. Labeling reaction: Amine-PEG3-Biotin reacts with the target biological molecule, usually by amidation reaction to connect the dye molecule to the target molecule. Depending on the desired labeling effect, the reaction conditions, such as pH, reaction time and temperature, may need to be optimized.
2. Purification of labeled molecules: After the reaction is completed, unreacted biotin is usually removed by dialysis or chromatography.
3. Detection: Detection is performed using streptavidin-enzyme or streptavidin-fluorescent markers to amplify the signal for analysis.
2. Western Blot and immunoassay
1. Labeling antibodies: Enhance the detection sensitivity by labeling Amine-PEG3-Biotin with primary or secondary antibodies.
2. Detection: The labeled antibody is used for protein transfer and detection is performed using streptavidin-enzyme or streptavidin-fluorescent markers.
3. Signal amplification: The strong binding of biotin to streptavidin enables the signal to be amplified, improving the sensitivity of immunoassay.
III. ELISA (Enzyme-Linked Immunosorbent Assay)
1. Coating: Coat the biotin-labeled target molecule onto the ELISA plate (such as a labeled antibody or antigen).
2. Detection: Detection is performed by adding streptavidin-enzyme conjugates and generating color or signal through substrate reaction.
IV. Cell experiment
1. Labeling cells: Add Amine-PEG3-Biotin to the cell culture medium and incubate for a certain period of time (usually 30 minutes to 1 hour).
2. Washing: Wash away unbound dye with PBS.
3. Fluorescence microscopy: Use a fluorescence microscope or flow cytometer for detection to observe the staining pattern and dynamic process of the cells.

The above information is based on published literature. Experimental procedures should be appropriately modified to meet specific research demands.

Chemical Properties

Molecular Weight	418.55
Formula	C₁₈H₃₄N₄O₅S
Cas No.	359860-27-8
Smiles	C(CCCC(NCCOCCOCCOCCN)=O)[C@H]1[C@@]2([C@](CS1)(NC(=O)N2)[H])[H]
Relative Density.	1.166 g/cm3 (Predicted)

Storage & Solubility Information

Storage

keep away from direct sunlight | store at -20°C | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

H2O: 95 mg/mL (226.97 mM), Sonication is recommended.

DMSO: 245 mg/mL (585.35 mM), Sonication is recommended.

In Vivo Formulation

10% DMSO+40% PEG300+5% Tween-80+45% Saline: 1 mg/mL (2.39 mM), Sonication is recommended.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

H2O/DMSO

	1mg	5mg	10mg	50mg
1 mM	2.3892 mL	11.9460 mL	23.8920 mL	119.4600 mL
5 mM	0.4778 mL	2.3892 mL	4.7784 mL	23.8920 mL
10 mM	0.2389 mL	1.1946 mL	2.3892 mL	11.9460 mL
20 mM	0.1195 mL	0.5973 mL	1.1946 mL	5.9730 mL
50 mM	0.0478 mL	0.2389 mL	0.4778 mL	2.3892 mL
100 mM	0.0239 mL	0.1195 mL	0.2389 mL	1.1946 mL

Calculator

Molarity Calculator
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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

Keywords

InhibitorinhibitAmine-PEG3-BiotinAmine-PEG-3-BiotinAminePEG3BiotinAmine PEG3 Biotin