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alpha-Amanitin

Catalog No. T13540 CAS 23109-05-9

Synonyms: α-Amanitin, α-Amatoxin

alpha-Amanitin is the principal toxin of poisonous mushrooms, exerting its toxic function by inhibiting RNA-polymerase II.

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alpha-Amanitin, CAS 23109-05-9

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1 mg	35 days	$ 1,128.00

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Biological Description

Chemical Properties

Storage & Solubility Information

Description	alpha-Amanitin is the principal toxin of poisonous mushrooms, exerting its toxic function by inhibiting RNA-polymerase II.
In vitro	Alpha-Amanitin decreases TAF15 mRNA and TAF15 protein levels in MKN45 cells and inhibits the RNAPII activity towards TAF15 mRNA [2]. alpha-Amanitin decreases cell viability by 14%, 21%, 41%, 44%, and 50% at concentrations of 100, 10, 1, 0.1, and 0.01 μg/mL, respectively. The LD50 of the alpha-Amanitin at 36 h is measured as 1 μg/mL. The total amount of protein within the cell at 24 h is significantly increased for the 1 μg/mL dose of alpha-Amanitin compared to the control [3]. Alpha-Amanitin dramatically decreases the expression of gap junctional genes and FSHr and LHR in cumulus cells [4].
In vivo	The intravenous lethal dose 50 (LD50) of alpha-Amanitin is determined to be 0.327 mg/kg in BALB/c mice. Following administration via the caudal vein, significant reductions in white blood cells (WBC), red blood cells (RBC), and hemoglobin (HGB) levels are observed at 12 hours, alongside notable increases in blood urea nitrogen (BUN) and creatinine (Crea) levels in serum. Alpha-Amanitin also impacts gene expression, specifically inhibiting genes (Irx4, Hsp90b1, etc.) involved in regulating RNA polymerase II activity and downregulating proteins (Nmi, Trpc5, etc.) associated with transcription processes [1]. Its efficacy extends to suppressing disseminated tumor cells (DTC), demonstrated by the stabilized body weight of mice injected with alpha-Amanitin (0.4?mg/kg, i.p.) treated cells, contrary to the weight loss seen in mice injected peritoneally with MKN45 cells [2].

Synonyms	α-Amanitin, α-Amatoxin
Molecular Weight	918.97
Formula	C39H54N10O14S
CAS No.	23109-05-9

Storage

store at low temperature

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

Solubility Information

H2O: 33.33 mg/mL (36.27 mM)

References and Literature

1. Zhao J, et al. Pathological effects of the mushroom toxin alpha-amanitin on BALB/c mice. Peptides. 2006 Dec;27(12):3047-3052. 2. Kume K, et al. α-Amanitin Restrains Cancer Relapse from Drug-Tolerant Cell Subpopulations via TAF15. Sci Rep. 2016 May 16;6:25895 3. Kaya E, et al. Evaluation and comparison of alpha- and beta-amanitin toxicity on MCF-7 cell line. Turk J Med Sci. 2014;44(5):728-32 4. Park MW, et al. RNA Polymerase II Inhibitor, α-Amanitin, Affects Gene Expression for Gap Junctions and Metabolic Capabilities of Cumulus Cells, but Not Oocyte, during in vitro Mouse Oocyte Maturation. Dev Reprod. 2013 Mar;17(1):63-72

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc.

Keywords

alpha-Amanitin 23109-05-9 Others alphaAmanitin alpha-Amatoxin α-Amanitin a-Amanitin α-Amatoxin alpha Amanitin a-Amatoxin inhibitor inhibit

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