ADDA 5 hydrochloride is a specific and non-competitive inhibitor of CcO (cytochrome c oxidase) that inhibits the proliferation of chemically sensitive and chemically resistant glioma cells and blocks cell proliferation and neurosphere formation in glioma stem cell cultures.

CcO (human glioma):18.93 μM, CcO (bovine heart):31.82 μM

Method:
ADDA 5 hydrochloride was used to treat cytochrome c oxidase (CcO) purified from human glioma cells and bovine heart, as well as glioblastoma stem cell (GSC) lines derived from UTMZ and Jx22, to evaluate its inhibitory effects on CcO activity and cell proliferation.
Result:
ADDA 5 hydrochloride partially and non-competitively inhibited CcO from human glioma and bovine heart, with IC₅₀ values of 18.93 μM and 31.82 μM, respectively. It also inhibited CcO activity in UTMZ and Jx22 GSCs, with IC₅₀ values of 21.4 μM and 15.5 μM, and exhibited antiproliferative effects on UTMZ cells (EC₅₀ = 8.17 μM).[1]

Method:
ADDA 5 was administered intraperitoneally (i.p.) at a dose of 8 mg/kg to tumor-bearing mice to evaluate its antitumor efficacy and in vivo toxicity.

Result:
ADDA 5 (8 mg/kg, i.p.) significantly inhibited tumor growth in mice, and no detectable toxicity was observed at doses up to 80 mg/kg. [1]