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Trastuzumab

Catalog No. T9912   CAS 180288-69-1
Synonyms: Trastuzumab (anti-HER2), anti-HER2, Herceptin

Trastuzumab is a humanized monoclonal antibody for patients with invasive breast cancers that overexpress HER2. Trastuzumab has been clinically used to treat HER2 Positive Metastatic Breast Cancer and HER2 Positive Gastric Cancer.

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Trastuzumab, CAS 180288-69-1
Pack Size Availability Price/USD Quantity
1 mg In stock 157.00
5 mg In stock 535.00
25 mg In stock 2029.00
50 mg In stock 3244.00
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Purity 99.9%
Biological Description
Chemical Properties
Storage & Solubility Information
Description Trastuzumab is a humanized monoclonal antibody for patients with invasive breast cancers that overexpress HER2. Trastuzumab has been clinically used to treat HER2 Positive Metastatic Breast Cancer and HER2 Positive Gastric Cancer.
Targets&IC50 HER2
Cell Research
The effects of trastuzumab and trastuzumab-F(ab′)2 on the growth of JIMT-1, SKBR-3, and BT-474 cells are evaluated using the AlamarBlue method. Exponentially growing cells are harvested and plated in single wells of a 96-well flat-bottomed tissue culture plate at defined densities, ranging from 4,500-8,000 cells per well depending on the cell line. After overnight culture, the regular medium is exchanged to medium containing 0, 1, 10, or 100 μg/mL trastuzumab or trastuzumab-F(ab′) 2. Cell viability is tested after 72 h of treatment. Fluorescence is detected at an excitation of 544 nm, and emission is detected at 590 nm[3].
Cell lines: JIMT-1, SKBR-3, and BT-474
Animal Research
Trastuzumab and trastuzumab-F(ab′)2 are given at a dose of 5 and 25 μg/g, respectively, by weekly i.p. injection. The five times greater amount of administered F(ab′)2 is chosen based on the different half-lives of IgG and F(ab′) F(ab′)2. Control mice are treated with weekly i.p. injection of 100 μL physiologic saline (saline) or rituximab (a humanized anti-CD20 antibody) at a dose of 5 μg/g. Animals are euthanized by CO2 inhalation[3].
Synonyms Trastuzumab (anti-HER2) , anti-HER2 , Herceptin
Purity 99.9%
Molecular Weight 146000
CAS No. 180288-69-1

Storage

Store the unopened solution at -20℃, and store it at 4℃ after melting to avoid repeated freeze-thaw

Solubility Information

( < 1 mg/ml refers to the product slightly soluble or insoluble )

Citations

References and Literature
1. Sliwkowski MX, et al. Nonclinical studies addressing the mechanism of action of trastuzumab (Herceptin). Semin Oncol. 1999 Aug;26(4 Suppl 12):60-70. 2. Hudis CA, et al. Trastuzumab--mechanism of action and use in clinical practice. N Engl J Med. 2007 Jul 5;357(1):39-51. 3. Barok M, et al. Trastuzumab causes antibody-dependent cellular cytotoxicity-mediated growth inhibition of submacroscopic JIMT-1 breast cancer xenografts despite intrinsic drug resistance. Mol Cancer Ther. 2007 Jul;6(7):2065-72.

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