Store the unopened solution at -20℃, and store it at 4℃ after melting to avoid repeated freeze-thaw
Trastuzumab is a humanized monoclonal antibody for patients with invasive breast cancers that overexpress HER2. Trastuzumab has been clinically used to treat HER2 Positive Metastatic Breast Cancer and HER2 Positive Gastric Cancer.
Pack Size | Availability | Price/USD | Quantity |
---|---|---|---|
1 mg | In stock | 157.00 | |
5 mg | In stock | 535.00 | |
25 mg | In stock | 2029.00 | |
50 mg | In stock | 3244.00 |
Description | Trastuzumab is a humanized monoclonal antibody for patients with invasive breast cancers that overexpress HER2. Trastuzumab has been clinically used to treat HER2 Positive Metastatic Breast Cancer and HER2 Positive Gastric Cancer. |
Targets&IC50 | HER2 |
Cell Research |
The effects of trastuzumab and trastuzumab-F(ab′)2 on the growth of JIMT-1, SKBR-3, and BT-474 cells are evaluated using the AlamarBlue method. Exponentially growing cells are harvested and plated in single wells of a 96-well flat-bottomed tissue culture plate at defined densities, ranging from 4,500-8,000 cells per well depending on the cell line. After overnight culture, the regular medium is exchanged to medium containing 0, 1, 10, or 100 μg/mL trastuzumab or trastuzumab-F(ab′) 2. Cell viability is tested after 72 h of treatment. Fluorescence is detected at an excitation of 544 nm, and emission is detected at 590 nm[3].
Cell lines: JIMT-1, SKBR-3, and BT-474
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Animal Research |
Trastuzumab and trastuzumab-F(ab′)2 are given at a dose of 5 and 25 μg/g, respectively, by weekly i.p. injection. The five times greater amount of administered F(ab′)2 is chosen based on the different half-lives of IgG and F(ab′) F(ab′)2. Control mice are treated with weekly i.p. injection of 100 μL physiologic saline (saline) or rituximab (a humanized anti-CD20 antibody) at a dose of 5 μg/g. Animals are euthanized by CO2 inhalation[3].
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Synonyms | Trastuzumab (anti-HER2) , anti-HER2 , Herceptin |
Purity | 99.9% |
Molecular Weight | 146000 |
CAS No. | 180288-69-1 |
Store the unopened solution at -20℃, and store it at 4℃ after melting to avoid repeated freeze-thaw
( < 1 mg/ml refers to the product slightly soluble or insoluble )
You can also refer to dose conversion for different animals. More
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