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Ponceau S

Name: Ponceau S
Brand: TargetMol
SKU: T23173
Rating: 4.5 (1 reviews)

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Catalog No. T23173Cas No. 6226-79-5

Alias Acid Red 112

Ponceau S (Acid Red 112) is an azo protein dye commonly used as a stain in spot blotting assays for protein quantification.

Ponceau S

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Catalog No. T23173Alias Acid Red 112Cas No. 6226-79-5

Ponceau S (Acid Red 112) is an azo protein dye commonly used as a stain in spot blotting assays for protein quantification.

Pack Size	Price	USA Warehouse	Global Warehouse	Quantity
1 g	$36	7-10 days	7-10 days
5 g	$48	7-10 days	7-10 days

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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.

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Product Introduction

Bioactivity

Description	Ponceau S (Acid Red 112) is an azo protein dye commonly used as a stain in spot blotting assays for protein quantification.
In vitro	1. Use in Western Blot 1. Material preparation: 1) Ponceau S solution: generally prepared as a 0.1% solution, Ponceau S can be dissolved in water or 1% ethanol. 2) Transfer membrane: usually PVDF or nitrocellulose membrane, on which the protein in the sample is transferred. 3) Decontamination buffer (TBST or other suitable buffer): used to remove excess dye. 2. Steps: 1) Staining: Soak the transferred membrane in Ponceau S solution, usually staining for 5-10 minutes, until the protein band appears obvious red. 2) Washing: Wash the membrane with decontamination buffer to remove excess dye, usually washing several times until the background of the protein band is clearer. 3) Detection: Use a scanner or camera to shoot the stained membrane, observe the position and intensity of the protein band, and perform quantitative analysis. 2. Protein quantitative analysis By comparing the intensity of standard protein bands (such as β-actin, GAPDH, etc.), a preliminary estimate of the protein concentration in unknown samples can be made. The above information is based on published literature. Experimental procedures should be appropriately modified to meet specific research demands.
Synonyms	Acid Red 112

Chemical Properties

Molecular Weight	760.57
Formula	C₂₂H₁₂N₄Na₄O₁₃S₄
Cas No.	6226-79-5
Smiles	[Na+].[Na+].[Na+].[Na+].Oc1c(cc2cc(ccc2c1\N=N\c1ccc(cc1S([O-])(=O)=O)\N=N\c1ccc(cc1)S([O-])(=O)=O)S([O-])(=O)=O)S([O-])(=O)=O
Relative Density.	1.01 g/mL at 20°C

Storage & Solubility Information

Storage

keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

H2O: 20 mg/mL (26.3 mM), Sonication is recommended.

DMSO: 80 mg/mL (105.18 mM), Sonication is recommended.

Solution Preparation Table

H2O/DMSO

	1mg	5mg	10mg	50mg
1 mM	1.3148 mL	6.5740 mL	13.1480 mL	65.7402 mL
5 mM	0.2630 mL	1.3148 mL	2.6296 mL	13.1480 mL
10 mM	0.1315 mL	0.6574 mL	1.3148 mL	6.5740 mL
20 mM	0.0657 mL	0.3287 mL	0.6574 mL	3.2870 mL

DMSO

	1mg	5mg	10mg	50mg
50 mM	0.0263 mL	0.1315 mL	0.2630 mL	1.3148 mL
100 mM	0.0131 mL	0.0657 mL	0.1315 mL	0.6574 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

Keywords

WesternstainredproteinsPonceau S Staining SolutionPonceau Sblottingantibody-antigenacidicAcid Red-112Acid Red112