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O-Propargyl-Puromycin

Name: O-Propargyl-Puromycin
Brand: TargetMol
SKU: T12284
Price: 46 USD
Availability: InStock
Rating: 5 (10 reviews)

(Synonyms: O-Propargylpuromycin, OP-puro) Copy Product Info

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Synonyms: O-Propargylpuromycin, OP-puro

Catalog No. T12284 Copy Product Info

Purity: 99.45%

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O-Propargyl-Puromycin ( OP-puro) is a natural product derivative and a protein synthesis inhibitor with cell permeability. It terminates translational elongation by incorporation into nascent peptide chains, and is widely used for nascent protein labeling and detection, enabling fluorescence imaging or affinity enrichment when combined with click chemistry.

Cas No. 1416561-90-4

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Pack Size	Price	USA Stock	Global Stock
500 μg	$46	In Stock	In Stock
1 mg	$66	In Stock	In Stock
5 mg	$158	In Stock	In Stock
10 mg	$248	In Stock	In Stock
25 mg	$397	In Stock	In Stock
50 mg	$569	In Stock	In Stock
1 mL x 10 mM (in DMSO)	$175	In Stock	In Stock

For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)

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For research use only—not for human use. No sales to individuals. Use as intended only.

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Purity:99.45%

Appearance:Solid

Color:White

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COA LCMS HNMR ORP

Product Introduction

Bioactivity

Description	O-Propargyl-Puromycin ( OP-puro) is a natural product derivative and a protein synthesis inhibitor with cell permeability. It terminates translational elongation by incorporation into nascent peptide chains, and is widely used for nascent protein labeling and detection, enabling fluorescence imaging or affinity enrichment when combined with click chemistry.
In vitro	Methods: Reticulocyte lysates and 293T cells were treated with O-Propargyl-Puromycin (25 μM) for 1 hour, and protein synthesis inhibition was detected by SDS-PAGE and autoradiography. Nascent polypeptide labeling was assessed through copper-catalyzed click chemistry combined with affinity purification. Results: O-Propargyl-Puromycin inhibited protein synthesis with an efficacy of 1/2 to 1/3 that of puromycin, covalently bound to nascent peptide chains, and was rapidly degraded by the proteasome, making it applicable for imaging of nascent proteins in cells and tissues. [1]
In vivo	Methods: Intraperitoneal injection of O-Propargyl-Puromycin (20 mM, 100 μL, dissolved in PBS) was administered to 3-week-old mice. Tissues were harvested and fixed 1 hour post-injection, followed by CuAAC staining with TMR-azide. PBS-injected mice served as controls. Results: Strong staining was observed in the small intestinal crypts and Paneth cells of injected mice, with distinct labeling of secretory granules, confirming that O-Propargyl-Puromycin can specifically label nascent proteins in vivo. [1]
Synonyms	O-Propargylpuromycin, OP-puro

Chemical Properties

Molecular Weight	495.53
Formula	C₂₄H₂₉N₇O₅
Cas No.	1416561-90-4
Smiles	CN(C)c1ncnc2n(cnc12)[C@@H]1O[C@H](CO)[C@@H](NC(=O)[C@@H](N)Cc2ccc(OCC#C)cc2)[C@H]1O
Relative Density.	1.44 g/cm3 (Predicted)

Storage & Solubility Information

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 257.5 mg/mL (519.65 mM), Sonication is recommended.

In Vivo Formulation

10% DMSO+40% PEG300+5% Tween 80+45% Saline: 5 mg/mL (10.09 mM), Sonication is recommended.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	2.0180 mL	10.0902 mL	20.1804 mL	100.9021 mL
5 mM	0.4036 mL	2.0180 mL	4.0361 mL	20.1804 mL
10 mM	0.2018 mL	1.0090 mL	2.0180 mL	10.0902 mL
20 mM	0.1009 mL	0.5045 mL	1.0090 mL	5.0451 mL
50 mM	0.0404 mL	0.2018 mL	0.4036 mL	2.0180 mL
100 mM	0.0202 mL	0.1009 mL	0.2018 mL	1.0090 mL

Note : The dilution table applies only to solid products. For liquid products, please calculate the stock solution based on the stated concentration and/or density.

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Keywords

protein synthesisO-Propargyl-PuromycinOPropargylPuromycinO Propargyl Puromycin

Related Tags: O-Propargyl-Puromycin chemical structure | O-Propargyl-Puromycin in vivo | O-Propargyl-Puromycin in vitro | O-Propargyl-Puromycin formula | O-Propargyl-Puromycin molecular weight