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NNMTi

Catalog No. T8760 CAS 42464-96-0

Synonyms: 5-Amino-1-methylquinolinium

NNMTi (5-Amino-1-methylquinolinium) is a substrate site-targeting, selective nicotinamide N-methyltransferase (NNMT) inhibitor (IC50 = 1.2 μM; 50 μM SAM, 100 μM NCA) that reduces 3T3-L1 lipogenesis (EC50 = 30μM) and adipocytes 1-methylnicotinamide level (EC50 = 2.3 μM) without affecting related methyltransferases & enzymes in the NAD+ salvage pathway

All products from TargetMol are for Research Use Only. Not for Human or Veterinary or Therapeutic Use.

NNMTi, CAS 42464-96-0

Pack Size	Availability	Price/USD
2 mg	In stock	$ 39.00
5 mg	In stock	$ 64.00
10 mg	In stock	$ 98.00
25 mg	In stock	$ 189.00
50 mg	In stock	$ 328.00
100 mg	In stock	$ 497.00
1 mL * 10 mM (in DMSO)	In stock	$ 70.00

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Purity: 99.93%

Biological Description

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Description

In vivo

NNMTi treatment will rescue age-related deficits in muSC activity to promote superior regeneration post-injury in aging muscle.?24-month old mice were treated with saline (control), and low and high dose NNMTi (5 and 10 mg/kg) for 1-week post-injury, or control and high dose NNMTi for 3-weeks post-injury.?All mice underwent an acute muscle injury (barium chloride injection) locally to the tibialis anterior (TA) muscle, and received 5-ethynyl-2'-deoxyuridine systemically to analyze muSC activity.?In vivo contractile function measurements were conducted on the injured TA muscle and tissues collected for ex-vivo analyses, including myofiber cross-sectional area (CSA) measurements to assess muscle recovery.?Results revealed that muscle stem cell proliferation and subsequent fusion were elevated in NNMTi-treated mice, supporting nearly 2-fold greater CSA and shifts in fiber size distribution to greater proportions of larger sized myofibers and fewer smaller sized fibers in NNMTi-treated mice compared to controls.?Prolonged NNMTi treatment post-injury further augmented myofiber regeneration evinced by increasingly larger fiber CSA.?Importantly, improved muSC activity translated not only to larger myofibers after injury but also to greater contractile function, with the peak torque of the TA increased by 70% in NNMTi-treated mice compared to controls.?Similar results were recapitulated in vitro with C2C12 myoblasts, where NNMTi treatment promoted and enhanced myoblast differentiation with supporting changes in the cellular NAD+/NADH redox states[1].

Synonyms	5-Amino-1-methylquinolinium
Molecular Weight	286.11
Formula	C10H11IN2
CAS No.	42464-96-0

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

Solubility Information

DMSO: 2.86 mg/mL (10.01 mM)

References and Literature

1. Harshini Neelakantan, et al. Small molecule nicotinamide N-methyltransferase inhibitor activates senescent muscle stem cells and improves regenerative capacity of aged skeletal muscle. Biochem Pharmacol. 2019 May;163:481-492. 2. Benjamin Trinité, Hongtao Zhang, David N Levy ,et al.NNRTI-induced HIV-1 protease-mediated cytotoxicity induces rapid death of CD4 T cells during productive infection and latency reversal.Retrovirology. 2019 Jun 26;16(1):17.

Related compound libraries

This product is contained In the following compound libraries：

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc.

Keywords

NNMTi 42464-96-0 Others inhibit fusion muSC muscle stem cell NNMT Inhibitor 5-Amino-1-methylquinolinium regenerative inhibitor

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