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Hydrastinine hydrochloride

Catalog No. T1607 CAS 4884-68-8

Hydrastinine hydrochloride is a semisynthetic alkaloid from the hydrolysis of the alkaloid Hydrastine, used as a haemostatic drug.

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Hydrastinine hydrochloride, CAS 4884-68-8

Pack Size	Availability	Price/USD
10 mg	Inquiry	$ 100.00
25 mg	Inquiry	$ 180.00
50 mg	Inquiry	$ 310.00
100 mg	Inquiry	$ 510.00
1 mL * 10 mM (in DMSO)	Inquiry	$ 90.00

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Purity: 98%

Biological Description

Chemical Properties

Storage & Solubility Information

Description

Hydrastinine hydrochloride is a semisynthetic alkaloid from the hydrolysis of the alkaloid Hydrastine, used as a haemostatic drug.

Kinase Assay

Cyclic AMP-dependent protein kinase activity is assayed in a reaction mixture containing, in a final volume of 0.2 mL, 50 mM Tris-HCl (pH 7.0), 10 mM magnesium acetate, 2 mM EGTA, 1 μM cyclic AMP or absence of cyclic AMP, 3.3 to 20 μM [r-32P] ATP (4×105 c.p.m.), 0.5 μg of the enzyme, 100 μg of histone H2B and compound. The mixture is incubated at 30°C for 5 min. The reaction is terminated by adding 1mL of ice-cold 20% trichloroacetic acid after adding 500 μg of bovine serum albumin as a carrier protein. The sample is centrifuged at 3000 r.p.m. for 15min, the pellet is resuspended in ice-cold 10% trichloro-acetic acid solution and the centrifugation-resuspension cycle is repeated three times. The final pellet is dissolved in 1 mL of 1 N NaOH and radioactivity is measured with a liquid scintillation counter[1].

Molecular Weight	243.69
Formula	C11H14ClNO3
CAS No.	4884-68-8

Storage

Powder: -20°C for 3 years

In solvent: -80°C for 2 years

Solubility Information

H2O: 10 mM

( < 1 mg/ml refers to the product slightly soluble or insoluble )

References and Literature

1. POE CF, JOHNSON CC. Acta Pharmacol Toxicol (Copenh). 1954; 10(4):338-46.

Dose Conversion

You can also refer to dose conversion for different animals. More

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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO (Master liquid concentration mg/mL)，Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.

Method for preparing in vivo formulation：Take μL DMSO master liquid, next add μL PEG300， mix and clarify, next add μL Tween 80，mix and clarify, next add μL ddH₂O, mix and clarify.

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Be sure to add the solvent(s) in order. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.

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What is the mass of compound required to make a 10 mM stock solution in 10 ml of water given that the molecular weight of the compound is 197.13 g/mol?
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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc.

Keywords

Hydrastinine hydrochloride 4884-68-8 Others Alkaloid inhibit Hydrastinine Inhibitor clotting haemostatic inhibitor

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