keep away from moisture
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
Ac-DEVD-AFC is a fluorogenic substrate
Pack Size | Availability | Price/USD | Quantity |
---|---|---|---|
1 mg | In stock | $ 47.00 | |
5 mg | In stock | $ 147.00 | |
10 mg | In stock | $ 277.00 | |
25 mg | In stock | $ 469.00 | |
50 mg | In stock | $ 675.00 | |
100 mg | In stock | $ 942.00 | |
1 mL * 10 mM (in DMSO) | In stock | $ 258.00 |
Description | Ac-DEVD-AFC is a fluorogenic substrate |
In vitro | Caspase activation was measured using the fluorogenic compound N-acetyl-asp-glu-val-asp-7-amino-4-trifluoromethyl coumarin (Ac-DEVD-AFC). We show that this substrate rapidly enters cells where it is efficiently cleaved at the aspartate residue by specific caspases, yielding the fluorescent compound 7-amino-4-trifluoromethyl coumarin (AFC). Following cell disruption, released AFC was separated on HPLC and detected by fluorescence. The appearance of AFC in cells was blocked by the pancaspase inhibitor benzyloxycarbonyl-val-ala-asp-fluoromethylketone, thus establishing that intracellular caspases were responsible for the cleavage. Caspase activity was first noted after about 2 h of incubation with doxorubicin or dactinomycin, the production of AFC being linear with time afterward[1]. |
Molecular Weight | 729.61 |
Formula | C30H34F3N5O13 |
CAS No. | 201608-14-2 |
keep away from moisture
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
DMSO: 40 mg/mL (54.82 mM)
H2O: insoluble
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Ac-DEVD-AFC 201608-14-2 Others inhibit Inhibitor Ac DEVD AFC AcDEVDAFC inhibitor