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3-Methyltoxoflavin

Name: 3-Methyltoxoflavin
Brand: TargetMol
SKU: T5453
Price: 46 USD
Availability: InStock
Rating: 5 (10 reviews)

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Catalog No. T5453Cas No. 32502-62-8

Alias 3-methyl toxoflavin

3-Methyltoxoflavin (3-methyl toxoflavin) is an effective inhibitor of protein disulfide isomerase (PDI) and its IC50 value is 170 nM.

3-Methyltoxoflavin

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Purity: 99.81%

Catalog No. T5453Alias 3-methyl toxoflavinCas No. 32502-62-8

3-Methyltoxoflavin (3-methyl toxoflavin) is an effective inhibitor of protein disulfide isomerase (PDI) and its IC50 value is 170 nM.

Pack Size	Price	USA Warehouse	Global Warehouse
1 mg	$46	In Stock	In Stock
5 mg	$108	In Stock	In Stock
10 mg	$163	In Stock	In Stock
25 mg	$293	In Stock	In Stock
50 mg	$425	In Stock	In Stock
100 mg	$619	In Stock	In Stock
1 mL x 10 mM (in DMSO)	$119	In Stock	In Stock

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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.

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Purity:99.81%

Appearance:Solid

Color:Yellow

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COA HNMR LCMS

Product Introduction

Bioactivity

Description	3-Methyltoxoflavin (3-methyl toxoflavin) is an effective inhibitor of protein disulfide isomerase (PDI) and its IC50 value is 170 nM.
Targets&IC50	PDI:170 nM
In vitro	3-Methyltoxoflavin is an effective inhibitor of Protein disulfide isomerase (PDI), exhibiting significant potency with an IC50 value of 170 nM, and demonstrating marked toxicity across various human glioblastoma cell lines. It stands out as the most potent PDI inhibitor identified in screening efforts. Research involving Bromouridine labeling and sequencing (Bru-seq) has shown that 3-Methyltoxoflavin activates the Nrf2 antioxidant response, triggers ER stress response, and promotes autophagy. It achieves this by increasing the transcription and protein levels of heme oxygenase 1 and SLC7A11, while simultaneously downregulating expression of PDI target genes, such as TXNIP and EGR1. Notably, the cell death induced by 3-Methyltoxoflavin does not occur through traditional pathways like apoptosis or necrosis, but rather through a combination of autophagy and ferroptosis.
Synonyms	3-methyl toxoflavin

Chemical Properties

Molecular Weight	207.19
Formula	C₈H₉N₅O₂
Cas No.	32502-62-8
Smiles	Cc1nn(C)c2nc(=O)n(C)c(=O)c2n1
Relative Density.	1.56 g/cm3 (Predicted)

Storage & Solubility Information

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 150 mg/mL (723.97 mM), Sonication is recommended.

In Vivo Formulation

10% DMSO+40% PEG300+5% Tween 80+45% Saline: 5 mg/mL (24.13 mM), Sonication is recommended.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	4.8265 mL	24.1324 mL	48.2649 mL	241.3244 mL
5 mM	0.9653 mL	4.8265 mL	9.6530 mL	48.2649 mL
10 mM	0.4826 mL	2.4132 mL	4.8265 mL	24.1324 mL
20 mM	0.2413 mL	1.2066 mL	2.4132 mL	12.0662 mL
50 mM	0.0965 mL	0.4826 mL	0.9653 mL	4.8265 mL
100 mM	0.0483 mL	0.2413 mL	0.4826 mL	2.4132 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc