3-IN-PP1, a potent protein kinase D (PKD) inhibitor, exhibits pan-PKD inhibitory activity with IC50 values of 108 nM for PKD1, 94 nM for PKD2, and 108 nM for PKD3. Additionally, it serves as a broad-spectrum anticancer agent by inhibiting the growth of various tumor cells. 3-IN-PP1 is utilized in cancer research [1].

PKD1:108 nM, PKD2:94 nM, PKD3:108 nM

3-IN-PP1 is a potent inhibitor of the protein kinase D (PKD) family, including PKD1, PKD2, and PKD3, demonstrating IC50 values of 108, 94, and 108 nM, respectively [1]. At a concentration of 5 µM over a period of 0-114 hours, it exhibits strong anti-proliferative effects on PANC-1 cells, significantly inhibiting their proliferation after 96 and 144 hours of treatment [1]. Furthermore, with a concentration of 20 µM applied for 1 hour, 3-IN-PP1 effectively impedes PKD-dependent phosphorylation of cortactin in PANC-1 cells, indicating its role in disrupting cellular processes crucial for cancer cell growth and metastasis [1]. Additionally, this compound has been found to inhibit the growth of various tumor cells, with IC50 values ranging from 1.6 to 39.2 µM [1]. These findings highlight 3-IN-PP1's potential as an effective agent in cancer research and therapy, particularly for treatments targeting the PKD pathway and related mechanisms in tumor cells.