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3α-Aminocholestane

Name: 3α-Aminocholestane
Brand: TargetMol
SKU: T14037
Price: 49 USD
Availability: InStock
Rating: 5 (10 reviews)

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Catalog No. T14037Cas No. 2206-20-4

Alias 5α-Cholestan-3α-amine

3α-Aminocholestane (5α-Cholestan-3α-amine) is a potent inhibitor of inositol-5'-phosphatase 1 (SHIP1) containing the SH2 structural domain (IC50 : 2.5 μM) for research and immune-related diseases.

3α-Aminocholestane

😃Good

Catalog No. T14037Alias 5α-Cholestan-3α-amineCas No. 2206-20-4

Pack Size	Price	USA Warehouse	Global Warehouse	Quantity
1 mg	$49	-	In Stock

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All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.

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Batch Information

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Purity:≥98.0%

Appearance:Solid

Color:White

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COA HNMR

Product Introduction

Bioactivity

Description	3α-Aminocholestane (5α-Cholestan-3α-amine) is a potent inhibitor of inositol-5'-phosphatase 1 (SHIP1) containing the SH2 structural domain (IC50 : 2.5 μM) for research and immune-related diseases.
Targets&IC50	SHIP1:2.5 μM
In vitro	MM cells were treated with increasing concentrations of 3α-Aminocholestane (3AC) for 48 hours. After cell lysis, proteins were separated by SDS-PAGE and probed with antibodies against SHIP1, SHIP2, and PTEN. The results indicate that treatment with3α-Aminocholestane (3AC)leads to proteasomal degradation of the SHIP1 protein[1].
In vivo	NOD/SCID/γcIL2R (NSG) mutant mice were inoculated with 1 × 10^7 MM cells (OPM2) via intraperitoneal injection. Six hours later, the mice received the first injection of 3α-Aminocholestane (3AC), followed by daily injections for the next 7 days, and subsequently, injections every two weeks for the following 15 weeks. The results indicate that treatment with 3α-Aminocholestane (3AC) inhibits the growth of MM cells in vivo.[1].
Synonyms	5α-Cholestan-3α-amine

Chemical Properties

Molecular Weight	387.68
Formula	C₂₇H₄₉N
Cas No.	2206-20-4
Smiles	[H][C@@]1(CC[C@@]2([H])[C@]3([H])CC[C@@]4([H])C[C@H](N)CC[C@]4(C)[C@@]3([H])CC[C@]12C)[C@H](C)CCCC(C)C
Relative Density.	0.930 g/cm3 (Predicted)

Storage & Solubility Information

Storage

store at low temperature | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: <0.1 mg/mL

Ethanol: 30 mg/mL (77.38 mM), Sonication is recommended.

Solution Preparation Table

Ethanol

	1mg	5mg	10mg	50mg
1 mM	2.5794 mL	12.8972 mL	25.7945 mL	128.9723 mL
5 mM	0.5159 mL	2.5794 mL	5.1589 mL	25.7945 mL
10 mM	0.2579 mL	1.2897 mL	2.5794 mL	12.8972 mL
20 mM	0.1290 mL	0.6449 mL	1.2897 mL	6.4486 mL
50 mM	0.0516 mL	0.2579 mL	0.5159 mL	2.5794 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc