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22-HDHA

Catalog No. T35997 Copy Product Info
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22-HDHA is an oxidation product of docosahexaenoic acid (DHA).

22-HDHA

Copy Product Info
🥰Excellent
Catalog No. T35997

22-HDHA is an oxidation product of docosahexaenoic acid (DHA).

22-HDHA
Cas No. 90780-46-4
Pack SizePriceUSA StockGlobal StockQuantity
25 μg$1,05035 days35 days
50 μg$1,96035 days35 days
100 μg$3,79035 days35 days
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Product Introduction

Bioactivity
Description
22-HDHA is an oxidation product of docosahexaenoic acid (DHA).
In vitro
In vitro, it is formed upon incubation of rat liver microsomes with DHA and NADPH and also by the human cytochrome P450 (CYP) isoform CYP4F3B in BTI-TN-5B1-4 microsomes. Serum levels of 22-HDHA increase following dietary DHA supplementation in humans.
Chemical Properties
Molecular Weight344.49
FormulaC22H32O3
Cas No.90780-46-4
SmilesC(/C=C\C/C=C\C/C=C\C/C=C\CCO)/C=C\C/C=C\CCC(O)=O
Storage & Solubility Information
StorageShipping with blue ice/Shipping at ambient temperature.
Solubility Information
DMF: Miscible
0.1 M Na2CO3: 2 mg/mL (5.81 mM), Sonication is recommended.
Ethanol: Miscible
PBS (pH 7.2): 0.8 mg/mL (2.32 mM), Sonication is recommended.
DMSO: Miscible
Solution Preparation Table
PBS (pH 7.2)/0.1 M Na2CO3
1mg5mg10mg50mg
1 mM2.9028 mL14.5142 mL29.0284 mL145.1421 mL
0.1 M Na2CO3
1mg5mg10mg50mg
5 mM0.5806 mL2.9028 mL5.8057 mL29.0284 mL
Note : The dilution table applies only to solid products. For liquid products, please calculate the stock solution based on the stated concentration and/or density.

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:
TargetMol | Animal experiments For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent 10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH2O , the resulting working solution concentration would be 2 mg/mL.
Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSOTargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSOTargetMol | reagent stock solution to 400 μL PEG300TargetMol | reagent and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH2OTargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.
All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.
1 Enter information below:
mg/kg
g
μL
2 Enter the in vivo formulation:
% DMSO
%
% Tween 80
% Saline/PBS/ddH2O

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

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