Midkine Protein, Human, Recombinant (His & Avi)

Midkine is a heparin-binding growth factor, originally reported as the product of a retinoic acid-responsive gene during embryogenesis, but currently viewed as a multifaceted factor contributing to both normal tissue homeostasis and disease development. Midkine is abnormally expressed at high levels in various human malignancies and acts as a mediator for the acquisition of critical hallmarks of cancer, including cell growth, survival, metastasis, migration, and angiogenesis.

Pack Size	Availability	Price/USD
100 μg	5 days	$ 465.00
500 μg	5 days	$ 1,850.00
1 mg	5 days	$ 3,100.00

Pack Size

Availability

Price/USD

Quantity

100 μg

5 days

$ 465.00

500 μg

5 days

$ 1,850.00

1 mg

5 days

$ 3,100.00

Description

Species	Human
Expression System	E. coli
Tag	N-His-Avi
Accession Number	P21741-1
Synonyms	MDK, MEK, MK1, MKARAP, ARAP, MK, NEGF2
Construction	Recombinant Human Midkine Protein is expressed from E.coli with His tag and Avi Tag at the N-Terminus. It contains Val21-Asp143.[Accession \|P21741-1]
Protein Purity	> 95% as determined by Tris-Bis PAGE
Molecular Weight	The protein has a predicted MW of 16.46 kDa same as Tris-Bis PAGE result.

Endotoxin	Less than 1EU per μg by the LAL method.
Formulation	Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability & Storage	-20 to -80°C for 12 months as supplied from date of receipt. -20 to -80°C for 3-6 months in unopened state after reconstitution. 2-8°C for 2-7 days after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Shipping	In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Research Background	Midkine is a heparin-binding growth factor, originally reported as the product of a retinoic acid-responsive gene during embryogenesis, but currently viewed as a multifaceted factor contributing to both normal tissue homeostasis and disease development. Midkine is abnormally expressed at high levels in various human malignancies and acts as a mediator for the acquisition of critical hallmarks of cancer, including cell growth, survival, metastasis, migration, and angiogenesis.