Transforming growth factor beta (TGFβ) is a multifunctional cytokine that regulates cell growth, differentiation, adhesion, migration and death dependent on cell type, developmental stage, or tissue conditions. There are three isoforms of TGFβ (TGFβ-1, -2 and -3). TGF-beta-3 is released from LAP by integrins: integrin-binding results in distortion of the LAP chain and subsequent release of the active TGF-beta-3. TGF-beta 3 binds with high affinity to TGF-beta RII, a type II serine/threonine kinase receptor. This receptor then phosphorylates and activates type I serine/threonine kinase receptors, TGF-beta RI or ALK-1, to modulate transcription through Smad phosphorylation.
Pack Size | Availability | Price/USD | Quantity |
---|---|---|---|
20 μg | 5 days | $ 900.00 | |
100 μg | 5 days | $ 2,480.00 |
Description | Transforming growth factor beta (TGFβ) is a multifunctional cytokine that regulates cell growth, differentiation, adhesion, migration and death dependent on cell type, developmental stage, or tissue conditions. There are three isoforms of TGFβ (TGFβ-1, -2 and -3). TGF-beta-3 is released from LAP by integrins: integrin-binding results in distortion of the LAP chain and subsequent release of the active TGF-beta-3. TGF-beta 3 binds with high affinity to TGF-beta RII, a type II serine/threonine kinase receptor. This receptor then phosphorylates and activates type I serine/threonine kinase receptors, TGF-beta RI or ALK-1, to modulate transcription through Smad phosphorylation. |
Species | Human |
Expression System | Human Cells |
Tag | N-8His-Avi |
Accession Number | P10600 |
Synonyms | Transforming growth factor β-3, Latent TGF-β3, TGF-β-3, TGFβ3, TGFB3, TGF-beta-3, Transforming growth factor beta-3 proprotein |
Amino Acid | Leu30-Ser390 |
Construction | Biotinylated Recombinant Human Transforming Growth Factor Beta-3 Proprotein is produced by our Mammalian expression system and the target gene encoding Leu30-Ser390 (Cys33Ser) is expressed with a 8His, Avi tag at the N-terminus. |
Protein Purity | Greater than 90% as determined by reducing SDS-PAGE. (QC verified) |
Molecular Weight | 12&37-45 KDa, reducing conditions |
Endotoxin | Less than 0.1 ng/µg (1 EU/µg) as determined by LAL test. |
Formulation | Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. |
Reconstitution | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
Stability & Storage |
Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
Shipping |
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature listed below. |
Research Background | Transforming growth factor beta (TGFβ) is a multifunctional cytokine that regulates cell growth, differentiation, adhesion, migration and death dependent on cell type, developmental stage, or tissue conditions. There are three isoforms of TGFβ (TGFβ-1, -2 and -3). TGF-beta-3 is released from LAP by integrins: integrin-binding results in distortion of the LAP chain and subsequent release of the active TGF-beta-3. TGF-beta 3 binds with high affinity to TGF-beta RII, a type II serine/threonine kinase receptor. This receptor then phosphorylates and activates type I serine/threonine kinase receptors, TGF-beta RI or ALK-1, to modulate transcription through Smad phosphorylation. |
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Please read the User Guide of Recombinant Proteins for more specific information.
Latent TGF-beta 3 Protein, Human, Recombinant (His & Avi), Biotinylated Transforming growth factor β-3 TGFB 3 Latent TGF-β3 TGF-β-3 TGFβ3 Transforming growth factor β-3 proprotein TGFB-3 TGFB3 TGF-beta-3 Transforming growth factor beta-3 proprotein TGFbeta3 TGF-b-3 Transforming growth factor b-3 proprotein recombinant recombinant-proteins proteins protein