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Synonyms: PIG61, NISBD2, mENA, LEGFR, HER1, ERBB1, ErbB, EGFR, EC 2.7.10.1, EC 2.7.10

| Pack Size | Price | USA Stock | Global Stock | Quantity |
|---|---|---|---|---|
| 5 μg | $51 | 7-10 days | 7-10 days | |
| 10 μg | $80 | 7-10 days | 7-10 days | |
| 20 μg | $129 | 7-10 days | 7-10 days | |
| 50 μg | $248 | 7-10 days | 7-10 days | |
| 100 μg | $418 | In Stock | - | |
| 200 μg | $757 | 7-10 days | 7-10 days | |
| 500 μg | $1,670 | 7-10 days | 7-10 days |
| Bioactivity | 1. Human EGFR, His Tag captured on CM5 Chip via Anti-His Antibody can bind Human EGF, No Tag with an affinity constant of 0.18 nM as determined in SPR assay.
2. Human EGFR, His Tag captured on CM5 Chip via anti-his antibody can bind Human TGF-alpha, hFc Tag with an affinity constant of 0.31 μM as determined in SPR assay. |
| Description | EGFR Protein, Human, Recombinant (His & Avi) is expressed in HEK293 mammalian cells with C-His-Avi tag. The predicted molecular weight is 71.5 kDa and the accession number is P00533-1. |
| Species | Human |
| Expression System | HEK293 Cells |
| Tag | C-His-Avi |
| Accession Number | P00533-1 |
| Construction | Leu25-Ser645 |
| Protein Purity | > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC |
| Endotoxin | < 1.0 EU/μg of the protein as determined by the LAL method. |
| Formulation | Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS (pH 7.4). Typically, 8% trehalose is incorporated as a protective agent before lyophilization. |
| Reconstitution | Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing. |
| Stability & Storage | Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
| Shipping | In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice. |
| Synonyms | PIG61, NISBD2, mENA, LEGFR, HER1, ERBB1, ErbB, EGFR, EC 2.7.10.1, EC 2.7.10 |
| Research Background |
| Molecular Weight | 71.5 kDa (predicted). Due to glycosylation, the protein migrates to 85-110 kDa based on Tris-Bis PAGE result. |
| Storage | Store at low temperature Lyophilized powder: -20~-80°C for 1 year | Solution: -80°C for 6 months |
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