The epidermal growth factor receptor is a transmembrane protein that is a receptor for members of the epidermal growth factor family of extracellular protein ligands. The epidermal growth factor receptor is a member of the ErbB family of receptors, a subfamily of four closely related receptor tyrosine kinases: EGFR, HER2/neu, Her 3 and Her 4. Receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses.
Pack Size | Availability | Price/USD | Quantity |
---|---|---|---|
100 μg | 5 days | $ 814.00 | |
500 μg | 5 days | $ 3,250.00 | |
1 mg | 5 days | $ 5,440.00 |
Description | The epidermal growth factor receptor is a transmembrane protein that is a receptor for members of the epidermal growth factor family of extracellular protein ligands. The epidermal growth factor receptor is a member of the ErbB family of receptors, a subfamily of four closely related receptor tyrosine kinases: EGFR, HER2/neu, Her 3 and Her 4. Receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses. |
Species | Human |
Expression System | HEK293 |
Tag | C-His-Avi |
Accession Number | P00533-1 |
Synonyms | PIG61, ERBB, LEGFR, mENA, EC 2.7.10.1, EGFR, EC 2.7.10, ERBB1, NISBD2, HER1 |
Construction | Recombinant Biotinylated Human EGFR/HER1 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus. It contains Leu25-Ser645.[Accession |P00533-1] |
Protein Purity | > 95% as determined by HPLC |
Molecular Weight | The protein has a predicted MW of 71.5 kDa. Due to glycosylation, the protein migrates to 90-120 kDa based on Tris-Bis PAGE result. |
Endotoxin | Less than 1EU per μg by the LAL method. |
Formulation | Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability & Storage |
-20 to -80°C for 12 months as supplied from date of receipt. -20 to -80°C for 3-6 months in unopened state after reconstitution. 2-8°C for 2-7 days after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Shipping |
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. |
Research Background | The epidermal growth factor receptor is a transmembrane protein that is a receptor for members of the epidermal growth factor family of extracellular protein ligands. The epidermal growth factor receptor is a member of the ErbB family of receptors, a subfamily of four closely related receptor tyrosine kinases: EGFR, HER2/neu, Her 3 and Her 4. Receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses. |
bottom
Please read the User Guide of Recombinant Proteins for more specific information.
EGFR Protein, Human, Recombinant (His & Avi), Biotinylated ERBB-1 NISBD-2 PIG61 ERBB LEGFR HER 1 mENA EC 2.7.10.1 EGFR EC 2.7.10 ERBB1 NISBD2 PIG 61 HER-1 PIG-61 NISBD 2 HER1 ERBB 1 recombinant recombinant-proteins proteins protein