EGFR Protein, Human, Recombinant (His & Avi), Biotinylated

Catalog No. TMPK-00427

EGFR Protein, Human, Recombinant (His & Avi), Biotinylated is expressed in HEK293 mammalian cells with C-His-Avi tag. The predicted molecular weight is 71.5 kDa and the accession number is P00533-1.

EGFR Protein, Human, Recombinant (His & Avi), Biotinylated

Catalog No. TMPK-00427

EGFR Protein, Human, Recombinant (His & Avi), Biotinylated is expressed in HEK293 mammalian cells with C-His-Avi tag. The predicted molecular weight is 71.5 kDa and the accession number is P00533-1.

Pack Size	Price	Availability
5 μg	$91	7-10 days
10 μg	$148	7-10 days
20 μg	$245	7-10 days
50 μg	$483	7-10 days
100 μg	$813	7-10 days
200 μg	$1,470	7-10 days
500 μg	$3,250	7-10 days

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Product Information

Biological Activity	Biotinylated Human EGFR, His Tag captured on CM5 Chip via Anti-his antibody can bind Human EGF, No Tag with an affinity constant of 0.62 nM as determined in SPR assay.
Description	EGFR Protein, Human, Recombinant (His & Avi), Biotinylated is expressed in HEK293 mammalian cells with C-His-Avi tag. The predicted molecular weight is 71.5 kDa and the accession number is P00533-1.
Species	Human
Expression System	HEK293 Cells
Tag	C-His-Avi
Accession Number	P00533-1
Synonyms	PIG61,NISBD2,mENA,LEGFR,HER1,ERBB1,ERBB,EGFR,EC 2.7.10.1,EC 2.7.10
Construction	Leu25-Ser645
Protein Purity	> 95% as determined by HPLC
Molecular Weight	71.5 kDa (predicted). Due to glycosylation, the protein migrates to 90-120 kDa based on Tris-Bis PAGE result.
Endotoxin	< 1 EU/μg by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS (pH 7.4). Typically, 8% trehalose is incorporated as a protective agent before lyophilization.
Reconstitution	Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
Stability & Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice.
Research Background	The epidermal growth factor receptor is a transmembrane protein that is a receptor for members of the epidermal growth factor family of extracellular protein ligands. The epidermal growth factor receptor is a member of the ErbB family of receptors, a subfamily of four closely related receptor tyrosine kinases: EGFR, HER2/neu, Her 3 and Her 4. Receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses.