EGFR vIII Protein, Human, Recombinant (His)

Name: EGFR vIII Protein, Human, Recombinant (His)
Brand: TargetMol
SKU: TMPJ-00648
Rating: 4.5 (1 reviews)

(Synonyms: Receptor tyrosine-protein kinase erbB-1, Proto-oncogene c-ErbB-1, Epidermal growth factor receptor, EGFR) Copy Product Info

Synonyms: Receptor tyrosine-protein kinase erbB-1, Proto-oncogene c-ErbB-1, Epidermal growth factor receptor, EGFR

Catalog No. TMPJ-00648 Copy Product Info

EGFR vIII Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with C-6xHis tag. The predicted molecular weight is 61-75 Kda and the accession number is NP_001333870.

Pack Size	Price	USA Stock	Global Stock
5 µg	$80	7-10 days	7-10 days
10 µg	$129	7-10 days	7-10 days
20 µg	$198	7-10 days	7-10 days
50 µg	$390	7-10 days	7-10 days
100 µg	$626	7-10 days	7-10 days
200 µg	$987	7-10 days	7-10 days
500 µg	$1,900	7-10 days	7-10 days
1 mg	$2,730	7-10 days	7-10 days

For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)

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For research use only—not for human use. No sales to individuals. Use as intended only.

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User Guide of Recombinant Proteins

Product Introduction

Bioactivity

Bioactivity	Loaded Anti-Human EGFR mAb on Protein A Biosensor, can bind Human EGFR vIII-His with an affinity constant of 2.55 nM as determined in BLI assay. (Regularly tested)
Description	EGFR vIII Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with C-6xHis tag. The predicted molecular weight is 61-75 Kda and the accession number is NP_001333870.
Species	Human
Expression System	HEK293 Cells
Tag	C-6xHis
Accession Number	NP_001333870
Amino Acid	Leu25-Ser378
Construction	Leu25-Ser378
Protein Purity	Greater than 95% as determined by reducing SDS-PAGE. Greater than 95% as determined by SEC-HPLC.
Endotoxin	< 0.1 ng/µg (1 EU/µg) as determined by LAL test.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4.
Reconstitution	Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
Synonyms	Receptor tyrosine-protein kinase erbB-1, Proto-oncogene c-ErbB-1, Epidermal growth factor receptor, EGFR
Research Background	The EGFR subfamily of receptor tyrosine kinases is composed of EGFR, ErbB2, ErbB3 and ErbB4. The EGFR shares 43% - 44% aa sequence identity with the ECD of human EGFR subfamily. All these family members are type I transmembrane glycoproteins with an extracellular ligand binding domain. The extracellular ligand binding domain is containing two cysteine-rich domains separated by a spacer region and a cytoplasmic domain containing a membrane-proximal tyrosine kinase domain. Ligand binding could induce EGFR homodimerization and heterodimerization with ErbB2, resulting in cell signaling, heterodimerization tyrosine phosphorylation and kinase activation. It can bind EGF, amphiregulin, TGF-alpha, betacellulin, epiregulin, HB-EGF, epigen, and so on. Its signaling regulates multiple biological functions including cell proliferation, differentiation, motility, and apoptosis. EGFR can also be recruited to form heterodimers with the ligand-activated ErbB3 or ErbB4. EGFR is overexpressed in different tumors. Several anti-cancer drugs use EGFR as target.

Chemical Properties

Molecular Weight

61-75 Kda (reducing condition)

Storage & Solubility Information

Shipping	In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
Storage	Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.