CD45RA Protein, Human, Recombinant (His)

Name: CD45RA Protein, Human, Recombinant (His)
Brand: TargetMol
SKU: TMPJ-00808
Rating: 4.5 (1 reviews)

(Synonyms: receptor-type tyrosine-protein phosphatase C, PTPRC, protein tyrosine phosphatase, receptor type, C, LY5, L-CA, LCA, CD45R, CD45 antigen, CD45, B220) Copy Product Info

Synonyms: receptor-type tyrosine-protein phosphatase C, PTPRC, protein tyrosine phosphatase, receptor type, C, LY5, L-CA, LCA, CD45R, CD45 antigen, CD45, B220

Catalog No. TMPJ-00808 Copy Product Info

CD45RA Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with C-6xHis tag. The predicted molecular weight is 100-135 KDa and the accession number is P08575-8.

Pack Size	Price	USA Stock	Global Stock
5 µg	$74	7-10 days	7-10 days
10 µg	$118	7-10 days	7-10 days
20 µg	$188	7-10 days	7-10 days
50 µg	$355	7-10 days	7-10 days
100 µg	$596	7-10 days	7-10 days
200 µg	$983	7-10 days	7-10 days
500 µg	$2,010	7-10 days	7-10 days
1 mg	$3,020	7-10 days	7-10 days

For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)

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For research use only—not for human use. No sales to individuals. Use as intended only.

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User Guide of Recombinant Proteins

Product Introduction

Bioactivity

Bioactivity	Activity has not been tested. It is theoretically active, but we cannot guarantee it. If you require protein activity, we recommend choosing the eukaryotic expression version first.
Description	CD45RA Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with C-6xHis tag. The predicted molecular weight is 100-135 KDa and the accession number is P08575-8.
Species	Human
Expression System	HEK293 Cells
Tag	C-6xHis
Accession Number	P08575-8
Amino Acid	Gln26Lys482
Construction	Gln26Lys482
Protein Purity	Greater than 95% as determined by reducing SDS-PAGE. (QC verified)
Endotoxin	< 0.1 ng/µg (1 EU/µg) as determined by LAL test.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4.
Reconstitution	Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
Synonyms	receptor-type tyrosine-protein phosphatase C, PTPRC, protein tyrosine phosphatase, receptor type, C, LY5, L-CA, LCA, CD45R, CD45 antigen, CD45, B220
Research Background	Protein tyrosine phosphatase, receptor type C (CD45), also known as PTPRC is a member of the protein tyrosine phosphatase (PTP) family which is known for its function to serve as signaling molecules and to regulate a variety of cellular processes such as cell proliferation, differentiation, mitotic cycle and oncogenic transformation. It is a variably glycosylated 180-220 kDa transmembrane protein that is abundantly expressed on all nucleated cells of hematopoietic origin. CD45 has several isoforms, expressed according to cell type, developmental stage and antigenic exposure. CD45 has been best studied in T cells, where it determines T cell receptor signaling thresholds. CD45 is moved into or out of the immunological synapse (IS) membrane microdomain depending on the relative influence of interaction with the extracellular galectin lattice or the intracellular actin cytoskeleton. Galectin interaction can be fine-tuned by varying usage of the heavily O-glycosylated spliced regions and sialylation of N-linked carbohydrates. Within the IS, CD45 dephosphorylates and negatively regulates the src family kinase, LCK. In other leukocytes, CD45 influences differentiation and links immunoreceptor signaling with cytokine secretion and cell survival, partially overlapping in function with DEP-1/CD148. CD45 deletion causes in severe immunodeficiency, while point mutations may be associated with autoimmune disorders.

Chemical Properties

Molecular Weight

100-135 KDa (reducing condition)

Storage & Solubility Information

Shipping	In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
Storage	Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.