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CD45R0 Protein, Human, Recombinant (His)

Catalog No. TMPJ-00410
Synonyms: CD45 antigen, L-CA, protein tyrosine phosphatase, receptor type, C, CD45, PTPRC, B220, LY5, receptor-type tyrosine-protein phosphatase C, CD45R, LCA

CD45R0 Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with C-6xHis tag. The predicted molecular weight is 80-120 KDa and the accession number is P08575-4.

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CD45R0 Protein, Human, Recombinant (His)
Pack Size Availability Price/USD Quantity
10 μg 5 days $ 118.00
50 μg 5 days $ 355.00
500 μg 5 days $ 2,010.00
1 mg 5 days $ 3,020.00
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Biological Description
Technical Params
Product Properties
Description CD45R0 Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with C-6xHis tag. The predicted molecular weight is 80-120 KDa and the accession number is P08575-4.
Species Human
Expression System HEK293 Cells
Tag C-6xHis
Accession Number P08575-4
Synonyms CD45 antigen, L-CA, protein tyrosine phosphatase, receptor type, C, CD45, PTPRC, B220, LY5, receptor-type tyrosine-protein phosphatase C, CD45R, LCA
Amino Acid Gln26­Lys416
Construction Gln26­Lys416
Protein Purity Greater than 95% as determined by reducing SDS-PAGE. (QC verified)
Molecular Weight 80-120 KDa (reducing condition)
Endotoxin < 0.1 ng/µg (1 EU/µg) as determined by LAL test.
Formulation Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4.
Reconstitution Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
Stability & Storage

Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted protein solutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.

Shipping

In general, Lyophilized powders are shipping with blue ice. Solutions are shipping with dry ice.

Research Background Protein tyrosine phosphatase, receptor type C (CD45), also known as PTPRC is a member of the protein tyrosine phosphatase (PTP) family which is known for its function to serve as signaling molecules and to regulate a variety of cellular processes such as cell proliferation, differentiation, mitotic cycle and oncogenic transformation. It is a variably glycosylated 180-220 kDa transmembrane protein that is abundantly expressed on all nucleated cells of hematopoietic origin. CD45 has several isoforms, expressed according to cell type, developmental stage and antigenic exposure. CD45 has been best studied in T cells, where it determines T cell receptor signaling thresholds. CD45 is moved into or out of the immunological synapse (IS) membrane microdomain depending on the relative influence of interaction with the extracellular galectin lattice or the intracellular actin cytoskeleton. Galectin interaction can be fine-tuned by varying usage of the heavily O-glycosylated spliced regions and sialylation of N-linked carbohydrates. Within the IS, CD45 dephosphorylates and negatively regulates the src family kinase, LCK. In other leukocytes, CD45 influences differentiation and links immunoreceptor signaling with cytokine secretion and cell survival, partially overlapping in function with DEP-1/CD148. CD45 deletion causes in severe immunodeficiency, while point mutations may be associated with autoimmune disorders.

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Keywords

CD45R0 Protein, Human, Recombinant (His) CD45 antigen L-CA protein tyrosine phosphatase, receptor type, C CD45 LY-5 CD-45 PTPRC CD 45 B 220 B220 LY 5 LY5 receptor-type tyrosine-protein phosphatase C CD45R LCA B-220 recombinant recombinant-proteins proteins protein

 

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