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CD3D & CD3E Heterodimer Protein, Cynomolgus, Recombinant (hFc & His & Flag)
(Synonyms: CD3d molecule, δ (CD3-TCR complex), CD3d molecule, delta (CD3-TCR complex)) Copy Product InfoSynonyms: CD3d molecule, δ (CD3-TCR complex), CD3d molecule, delta (CD3-TCR complex)
Catalog No. TMPY-06348 Copy Product Info
CD3D & CD3E Heterodimer Protein, Cynomolgus, Recombinant is expressed in HEK293 mammalian cells. The predicted molecular weight is 76.63 kDa and the accession number is Q95LI8&Q95LI5.
| Pack Size | Price | USA Stock | Global Stock | Quantity |
|---|---|---|---|---|
| 5 μg | $93 | 7-10 days | 7-10 days | |
| 10 μg | $152 | 7-10 days | 7-10 days | |
| 20 μg | $248 | 7-10 days | 7-10 days | |
| 50 μg | $498 | 7-10 days | 7-10 days | |
| 100 μg | $817 | 7-10 days | 7-10 days | |
| 200 μg | $1,330 | 7-10 days | 7-10 days | |
| 500 μg | $2,930 | 7-10 days | 7-10 days |
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Product Introduction
Bioactivity
Chemical Properties
Storage & Solubility Information
| Bioactivity | Immobilized SP34 Antibody at 2 μg/mL (100 μL/well) can bind Recombinant Cynomolgus CD3D & CD3E Heterodimer Protein (Fc-His & Fc-Flag Tag) (Cat#TMPY-06348), the EC50 is 4-12 ng/mL. |
| Description | CD3D & CD3E Heterodimer Protein, Cynomolgus, Recombinant is expressed in HEK293 mammalian cells. The predicted molecular weight is 76.63 kDa and the accession number is Q95LI8&Q95LI5. |
| Species | Cynomolgus |
| Expression System | HEK293 Cells |
| Tag | C-hFc & His & C-hFc & Flag |
| Accession Number | Q95LI8&Q95LI5 |
| Construction | A DNA sequence encoding the cynomolugs CD3D (Q95LI8-1)(Met1-Ala105) with the C-terminal his-tagged Fc region of human IgG1 at the C-terminus, constructed the plasmid 1; A DNA sequence encoding the cynomolgus CD3E (XP_015290838.1) (Met1-Asp117) was fused with the C-terminal flag-tagged Fc region of human IgG1 at the C-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the CD3D&CD3E heterodimer was purified. Predicted N terminal: Phe 22 & Gln 22 |
| Protein Purity | ≥ 95% as determined by SDS-PAGE. ≥ 95% as determined by SEC-HPLC. |
| Endotoxin | < 1.0 EU/μg of the protein as determined by the LAL method. |
| Formulation | Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization. |
| Reconstitution | A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information. |
| Stability & Storage | It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
| Shipping | In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice. |
| Synonyms | CD3d molecule, δ (CD3-TCR complex), CD3d molecule, delta (CD3-TCR complex) |
| Molecular Weight | 76.63 kDa (predicted); (50.99 & 40.83) kDa (reducing conditions) |
| Storage | Lyophilized powder: -20~-80°C for 1 year | Solution: -80°C for 6 months |
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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Related Tags: CD3D & CD3E Heterodimer Protein, Cynomolgus, Recombinant (hFc & His & Flag) chemical structure | CD3D & CD3E Heterodimer Protein, Cynomolgus, Recombinant (hFc & His & Flag) molecular weight
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