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CD3D & CD3E Heterodimer Protein, Cynomolgus, Recombinant (hFc & His & Flag)

(Synonyms: CD3d molecule, δ (CD3-TCR complex), CD3d molecule, delta (CD3-TCR complex)) Copy Product Info

Synonyms: CD3d molecule, δ (CD3-TCR complex), CD3d molecule, delta (CD3-TCR complex)

Catalog No. TMPY-06348 Copy Product Info
CD3D & CD3E Heterodimer Protein, Cynomolgus, Recombinant is expressed in HEK293 mammalian cells. The predicted molecular weight is 76.63 kDa and the accession number is Q95LI8&Q95LI5.

Product Introduction

Bioactivity
Bioactivity
Immobilized SP34 Antibody at 2 μg/mL (100 μL/well) can bind Recombinant Cynomolgus CD3D & CD3E Heterodimer Protein (Fc-His & Fc-Flag Tag) (Cat#TMPY-06348), the EC50 is 4-12 ng/mL.
Description
CD3D & CD3E Heterodimer Protein, Cynomolgus, Recombinant is expressed in HEK293 mammalian cells. The predicted molecular weight is 76.63 kDa and the accession number is Q95LI8&Q95LI5.
Species
Cynomolgus
Expression System
HEK293 Cells
TagC-hFc & His & C-hFc & Flag
Accession NumberQ95LI8&Q95LI5
ConstructionA DNA sequence encoding the cynomolugs CD3D (Q95LI8-1)(Met1-Ala105) with the C-terminal his-tagged Fc region of human IgG1 at the C-terminus, constructed the plasmid 1; A DNA sequence encoding the cynomolgus CD3E (XP_015290838.1) (Met1-Asp117) was fused with the C-terminal flag-tagged Fc region of human IgG1 at the C-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the CD3D&CD3E heterodimer was purified. Predicted N terminal: Phe 22 & Gln 22
Protein Purity
≥ 95% as determined by SDS-PAGE. ≥ 95% as determined by SEC-HPLC.
Endotoxin< 1.0 EU/μg of the protein as determined by the LAL method.
FormulationLyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
ReconstitutionA Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
SynonymsCD3d molecule, δ (CD3-TCR complex), CD3d molecule, delta (CD3-TCR complex)
Chemical Properties
Molecular Weight76.63 kDa (predicted); (50.99 & 40.83) kDa (reducing conditions)
Storage & Solubility Information
ShippingIn general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
StorageIt is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.

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Related Tags: CD3D & CD3E Heterodimer Protein, Cynomolgus, Recombinant (hFc & His & Flag) chemical structure | CD3D & CD3E Heterodimer Protein, Cynomolgus, Recombinant (hFc & His & Flag) molecular weight