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CD28 Protein, Mouse, Recombinant (His), Biotinylated

(Synonyms: CD28 molecule) Copy Product Info

Synonyms: CD28 molecule

Catalog No. TMPY-05652 Copy Product Info
CD28 Protein, Mouse, Recombinant (His), Biotinylated is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 16.5 kDa and the accession number is P31041.

Product Introduction

Bioactivity
Bioactivity
Description
CD28 Protein, Mouse, Recombinant (His), Biotinylated is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 16.5 kDa and the accession number is P31041.
Species
Mouse
Expression System
HEK293 Cells
TagC-His
Accession NumberP31041
ConstructionA DNA sequence encoding the extracellular domain (Met 1-Lys 149) of mouse CD28 (NP_031668.3) was fused with a polyhistidine tag at the C-terminus. The purified protein was biotinylated in vitro. Predicted N terminal: Cys 27
Protein Purity
> 95 % as determined by SDS-PAGE
Endotoxin< 1.0 EU/μg of the protein as determined by the LAL method.
FormulationLyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
ReconstitutionA Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Stability & StorageIt is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
ShippingIn general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
SynonymsCD28 molecule
Research Background
Chemical Properties
Molecular Weight16.5 kDa (predicted); 32-35 kDa (reducing condition, due to glycosylation)
Storage & Solubility Information
StorageLyophilized powder: -20~-80°C for 1 year | Solution: -80°C for 6 months Shipping with blue ice/Shipping at ambient temperature.

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
Related Tags: CD28 Protein, Mouse, Recombinant (His), Biotinylated chemical structure | CD28 Protein, Mouse, Recombinant (His), Biotinylated molecular weight