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X-NeuNAc

Name: X-NeuNAc
Brand: TargetMol
SKU: T21486
Price: 36 USD
Availability: InStock
Rating: 5 (10 reviews)

(Synonyms: X-Neu5Ac, 5-Bromo-4-chloro-3-indolyl-alpha-D-N-acetylneuraminic acid sodium salt) Copy Product Info

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Synonyms: X-Neu5Ac, 5-Bromo-4-chloro-3-indolyl-alpha-D-N-acetylneuraminic acid sodium salt

Catalog No. T21486 Copy Product Info

Purity: 99.99%

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X-NeuNAc (5-Bromo-4-chloro-3-indolyl-alpha-D-N-acetylneuraminic acid sodium salt) is a new chromogenic assay substrate of neuraminidase activity in bacterial expression systems.

Cas No. 160369-85-7

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Pack Size	Price	USA Stock	Global Stock
5 mg	$36	In Stock	In Stock
10 mg	$55	In Stock	In Stock
25 mg	$98	In Stock	In Stock
50 mg	$168	In Stock	In Stock
100 mg	$255	In Stock	In Stock
1 mL x 10 mM (in DMSO)	$53	In Stock	In Stock

For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)

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For research use only—not for human use. No sales to individuals. Use as intended only.

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Purity:99.99%

Appearance:Solid

Color:White

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COA HNMR LCMS

Product Introduction

Bioactivity

Description	X-NeuNAc (5-Bromo-4-chloro-3-indolyl-alpha-D-N-acetylneuraminic acid sodium salt) is a new chromogenic assay substrate of neuraminidase activity in bacterial expression systems.
In vitro	X-Neu5Ac was hydrolyzed by neuraminidase to release a halogenated product undergoing rapid aerobic oxidation to form the dark blue pigment. Preliminary kinetic studies indicated that X-Neu5Ac was a good and stable substrate for neuraminidase. In addition, X-Neu5Ac would also be hydrolyzed mutant enzymes
In vivo	To visualize extracellular sialidase activity on the membrane surface in the rat brain, acute brain slices were incubated with X-NeuNAc at pH 7.3. After 1h, myelin-abundant regions showed intense fluorescence in the rat brain. Although the hippocampus showed weak fluorescence in the brain, mossy fiber terminals in the hippocampus showed relatively intense fluorescence. In addition, the fluorescence intensities caused by X-NeuNAc was correlated with the sialidase activity. Therefore, staining with X-NeuNAc was specific for sialidase and useful for quantitative analysis of sialidase activities [1].
Synonyms	X-Neu5Ac, 5-Bromo-4-chloro-3-indolyl-alpha-D-N-acetylneuraminic acid sodium salt

Chemical Properties

Molecular Weight	559.72
Formula	C₁₉H₂₁BrClN₂NaO₉
Cas No.	160369-85-7
Smiles	[Na+].CC(=O)N[C@@H]1[C@@H](O)C[C@@](Oc2c[nH]c3ccc(Br)c(Cl)c23)(O[C@H]1[C@H](O)[C@H](O)CO)C([O-])=O
Relative Density.	no data available

Storage & Solubility Information

Storage

Store at low temperature Powder: -20°C for 3 years | In solvent: -80°C for 1 year Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 250 mg/mL (446.65 mM), Sonication is recommended.

In Vivo Formulation

10% DMSO+90% Saline: 10 mg/mL (17.87 mM), Solution.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	1.7866 mL	8.9330 mL	17.8661 mL	89.3304 mL
5 mM	0.3573 mL	1.7866 mL	3.5732 mL	17.8661 mL
10 mM	0.1787 mL	0.8933 mL	1.7866 mL	8.9330 mL
20 mM	0.0893 mL	0.4467 mL	0.8933 mL	4.4665 mL
50 mM	0.0357 mL	0.1787 mL	0.3573 mL	1.7866 mL
100 mM	0.0179 mL	0.0893 mL	0.1787 mL	0.8933 mL

Note : The dilution table applies only to solid products. For liquid products, please calculate the stock solution based on the stated concentration and/or density.

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 µL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 µL Tween 80 and mix well until fully clarified.

3) Add 450 µL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc