Tubulin polymerization-IN-61

Name: Tubulin polymerization-IN-61
Brand: TargetMol
SKU: T87579
Rating: 4.5 (1 reviews)

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Catalog No. T87579Cas No. 2758114-60-0

Tubulin polymerization-IN-61 (Compound 9a), a tubulin polymerization inhibitor, disrupts the microtubule skeleton, arrests the cell cycle at the G2/M phase, induces Apoptosis, and impedes cancer cell migration and colony formation. This compound demonstrates antitumor efficacy in vivo within the 4T1 xenograft model [1].

Tubulin polymerization-IN-61

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Catalog No. T87579Cas No. 2758114-60-0

Pack Size	Price	USA Warehouse	Global Warehouse	Quantity
10 mg	Inquiry	10-14 weeks	10-14 weeks
50 mg	Inquiry	10-14 weeks	10-14 weeks

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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

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Product Introduction

Bioactivity

Description	Tubulin polymerization-IN-61 (Compound 9a), a tubulin polymerization inhibitor, disrupts the microtubule skeleton, arrests the cell cycle at the G2/M phase, induces Apoptosis, and impedes cancer cell migration and colony formation. This compound demonstrates antitumor efficacy in vivo within the 4T1 xenograft model [1].
In vitro	Tubulin polymerization-IN-61 exhibits antiproliferative activity against three human cancer cell lines (MCF-7, SGC-7901, and A549) with IC50 values ranging from 14 to 45 nM [1]. At concentrations of 20-60 nM for 24 hours, Tubulin polymerization-IN-61 increases the G2/M phase proportion of MCF-7 cells from 14% to 50%, induces apoptosis, and inhibits migration and colony formation of MCF-7 cells [1].
In vivo	Tubulin polymerization-IN-61, administered intravenously at doses of 5 or 15 mg/kg every other day for two weeks, demonstrates antitumor activity with negligible toxicity in the 4T1 xenograft tumor model in BALB/c mice [1].

Chemical Properties

Molecular Weight	407.42
Formula	C₂₂H₂₁N₃O₅
Cas No.	2758114-60-0
Smiles	O(C)C=1C=C(N2C=3C(N=N2)=CC=C(C3)C4=CC(O)=C(OC)C=C4)C=C(OC)C1OC

Storage & Solubility Information

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice.

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc