Tubulin polymerization-IN-61 (Compound 9a), a tubulin polymerization inhibitor, disrupts the microtubule skeleton, arrests the cell cycle at the G2/M phase, induces Apoptosis, and impedes cancer cell migration and colony formation. This compound demonstrates antitumor efficacy in vivo within the 4T1 xenograft model [1].

Tubulin polymerization-IN-61 exhibits antiproliferative activity against three human cancer cell lines (MCF-7, SGC-7901, and A549) with IC50 values ranging from 14 to 45 nM [1]. At concentrations of 20-60 nM for 24 hours, Tubulin polymerization-IN-61 increases the G2/M phase proportion of MCF-7 cells from 14% to 50%, induces apoptosis, and inhibits migration and colony formation of MCF-7 cells [1].

Tubulin polymerization-IN-61, administered intravenously at doses of 5 or 15 mg/kg every other day for two weeks, demonstrates antitumor activity with negligible toxicity in the 4T1 xenograft tumor model in BALB/c mice [1].