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ONPG

Name: ONPG
Brand: TargetMol
SKU: T19497
Price: 31 USD
Availability: InStock
Rating: 5 (10 reviews)

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Catalog No. T19497Cas No. 369-07-3

Alias 2-Nitrophenyl β-D-galactopyranoside

ONPG (2-Nitrophenyl β-D-galactopyranoside) is a colorimetric and spectrophotometric substrate used to detect β-galactosidase activity.

ONPG

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Purity: 99.91%

Catalog No. T19497Alias 2-Nitrophenyl β-D-galactopyranosideCas No. 369-07-3

ONPG (2-Nitrophenyl β-D-galactopyranoside) is a colorimetric and spectrophotometric substrate used to detect β-galactosidase activity.

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1 g	$31	In Stock	In Stock

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Purity:99.91%

Appearance:Solid

Color:White

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COA HPLC HNMR

Product Introduction

Bioactivity

Description	ONPG (2-Nitrophenyl β-D-galactopyranoside) is a colorimetric and spectrophotometric substrate used to detect β-galactosidase activity.
Targets&IC50	β-gal:2.7±0.3 mM(Km), β-gal:464.7±7.8/s(kcat)
In vitro	The enzyme displays high hydrolysis ability for ONPG (100%) and moderate activity for its natural substrate lactose (25.7%). However, the hydrolysis ability of the enzyme towards all other chromogenic nitrophenyl analogues is very weak, indicating that Gal308 is a β-galactosidase with narrow substrate specificity. To investigate the kinetic parameters of recombinant enzyme, the Michaelis-Menten constants (Km), turnover numbers (kcat), and catalytic efficiencies (kcat/Km) of Gal308 for ONPG and lactose are determined. The kcat and Km values are 464.7±7.8 s-1 and 2.7±0.3 mM for ONPG, and 264.2±2.1 s-1 and 7.1±0.8 mM for lactose, respectively. The kcat/Km value of the enzyme for ONPG (172.1 s-1mM-1) is 4.6-fold higher than that for lactose (37.2 s-1mM-1), which clearly demonstrated that the catalytic efficiency of Gal308 for ONPG is much higher than that for lactose[1].
Synonyms	2-Nitrophenyl β-D-galactopyranoside

Chemical Properties

Molecular Weight	301.25
Formula	C₁₂H₁₅NO₈
Cas No.	369-07-3
Smiles	O([C@@H]1O[C@H](CO)[C@H](O)[C@H](O)[C@H]1O)C2=C(N(=O)=O)C=CC=C2
Relative Density.	1.3709 g/cm3 (Estimated)

Storage & Solubility Information

Storage

store at low temperature | Powder: -20°C for 3 years | In solvent: -80°C for 1 year

Solubility Information

H2O: 7.4 mg/mL (24.56 mM), Sonication is recommended.

Solution Preparation Table

H2O

	1mg	5mg	10mg	50mg
1 mM	3.3195 mL	16.5975 mL	33.1950 mL	165.9751 mL
5 mM	0.6639 mL	3.3195 mL	6.6390 mL	33.1950 mL
10 mM	0.3320 mL	1.6598 mL	3.3195 mL	16.5975 mL
20 mM	0.1660 mL	0.8299 mL	1.6598 mL	8.2988 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc