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NBD-F
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Catalog No. T19031Cas No. 29270-56-2
Alias 4-Fluoro-7-nitrobenzofurazan
NBD-F (4-Fluoro-7-nitrobenzofurazan) is a fluorescent derivatization agent used for amino acid analysis.
NBD-F
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Purity: 99.51%
Catalog No. T19031Alias 4-Fluoro-7-nitrobenzofurazanCas No. 29270-56-2
NBD-F (4-Fluoro-7-nitrobenzofurazan) is a fluorescent derivatization agent used for amino acid analysis.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 25 mg | $38 | In Stock | In Stock | |
| 50 mg | $53 | In Stock | In Stock | |
| 100 mg | $86 | In Stock | In Stock | |
| 1 mL x 10 mM (in DMSO) | $29 | In Stock | In Stock |
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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Batch Information
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Purity:99.51%
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Product Introduction
Bioactivity
Chemical Properties
| Description | NBD-F (4-Fluoro-7-nitrobenzofurazan) is a fluorescent derivatization agent used for amino acid analysis. |
| In vitro | NBD-F for quantification of colistin sulfate in rat plasma 1. Sample preparation: Colistin sulfate (including CLA and CLB) was extracted from rat plasma using a solid phase extraction (SPE) C18 column. 2. Reaction with NBD-F: The extracted colistin sulfate was reacted with 2 mM NBD-F and 10 mM boric acid buffer (pH 9.5). 3. Heating reaction: The reaction mixture was heated at 60°C for 5 minutes to promote the derivatization reaction. 4. HPLC analysis: The derivatization products were analyzed using reversed-phase high performance liquid chromatography (HPLC) combined with fluorescence detection. 5. Quantitative analysis: The peaks of CLA and CLB were quantified using appropriate standards. The lower limit of quantification for CLA and CLB was 0.05 μg/mL. |
| Synonyms | 4-Fluoro-7-nitrobenzofurazan |
| Molecular Weight | 183.1 |
| Formula | C6H2FN3O3 |
| Cas No. | 29270-56-2 |
| Smiles | [O-][N+](=O)C1=CC=C(F)C2=NON=C12 |
| Relative Density. | 1.682g/cm3 |
Storage & Solubility Information
| Storage | keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature. | |||||||||||||||||||||||||||||||||||
| Solubility Information | DMSO: 110 mg/mL (600.76 mM), Sonication is recommended. | |||||||||||||||||||||||||||||||||||
| In Vivo Formulation | 10% DMSO+40% PEG300+5% Tween-80+45% Saline: 3.3 mg/mL (18.02 mM), Sonication is recommeded. Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions. | |||||||||||||||||||||||||||||||||||
Solution Preparation Table | ||||||||||||||||||||||||||||||||||||
DMSO
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Calculator
In Vivo Formulation Calculator (Clear solution)
Please enter your animal experiment information in the following box and click Calculate to obtain the mother liquor preparation method and in vivo formula preparation method:
For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . A total of 10 animals were administered, and the formula you used is 5% 
DMSO+30% PEG300+5% Tween 80+60% Saline/PBS/ddH2O. So your working solution concentration is 2 mg/mL。Mother liquor preparation method: 2 mg of drug dissolved in 50 μL DMSO (mother liquor concentration of 40 mg/mL), if you need to configure a concentration that exceeds the solubility of the product, please contact us first.
(mother liquor concentration of 40 mg/mL), if you need to configure a concentration that exceeds the solubility of the product, please contact us first.Preparation method for in vivo formula: Take 50 μL DMSO main solution, add 300 μLPEG300 mix well and clarify, then add 50 more μL Tween 80, mix well and clarify, then add 600 more μLSaline/PBS/ddH2O mix well and clarify
main solution, add 300 μLPEG300 mix well and clarify, then add 50 more μL Tween 80, mix well and clarify, then add 600 more μLSaline/PBS/ddH2O mix well and clarifyFor Reference Only. Please develop an appropriate dissolution method based on your laboratory animals and route of administration.
Dose Conversion
You can also refer to dose conversion for different animals. More Dose Conversion
Tech Support
Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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