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NBD-F (4-Fluoro-7-nitrobenzofurazan) is a fluorescent derivatization agent used for amino acid analysis.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
25 mg | $38 | Backorder | |
50 mg | $53 | Backorder | |
100 mg | $86 | Backorder | |
1 mL x 10 mM (in DMSO) | $29 | Backorder |
Description | NBD-F (4-Fluoro-7-nitrobenzofurazan) is a fluorescent derivatization agent used for amino acid analysis. |
In vitro | NBD-F for quantification of colistin sulfate in rat plasma 1. Sample preparation: Colistin sulfate (including CLA and CLB) was extracted from rat plasma using a solid phase extraction (SPE) C18 column. 2. Reaction with NBD-F: The extracted colistin sulfate was reacted with 2 mM NBD-F and 10 mM boric acid buffer (pH 9.5). 3. Heating reaction: The reaction mixture was heated at 60°C for 5 minutes to promote the derivatization reaction. 4. HPLC analysis: The derivatization products were analyzed using reversed-phase high performance liquid chromatography (HPLC) combined with fluorescence detection. 5. Quantitative analysis: The peaks of CLA and CLB were quantified using appropriate standards. The lower limit of quantification for CLA and CLB was 0.05 μg/mL. |
Alias | 4-Fluoro-7-nitrobenzofurazan |
Molecular Weight | 183.1 |
Formula | C6H2FN3O3 |
Cas No. | 29270-56-2 |
Smiles | [O-][N+](=O)C1=CC=C(F)C2=NON=C12 |
Relative Density. | 1.682g/cm3 |
Storage | keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice./Shipping at ambient temperature. | |||||||||||||||||||||||||||||||||||
Solubility Information | DMSO: 110 mg/mL (600.76 mM), Sonication is recommended. | |||||||||||||||||||||||||||||||||||
Solution Preparation Table | ||||||||||||||||||||||||||||||||||||
DMSO
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