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NBD-F

Name: NBD-F
Brand: TargetMol
SKU: T19031
Price: 29 USD
Availability: InStock
Rating: 5 (10 reviews)

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Catalog No. T19031Cas No. 29270-56-2

Alias 4-Fluoro-7-nitrobenzofurazan

NBD-F (4-Fluoro-7-nitrobenzofurazan) is a fluorescent derivatization agent used for amino acid analysis.

NBD-F

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Purity: 99.51%

Catalog No. T19031Alias 4-Fluoro-7-nitrobenzofurazanCas No. 29270-56-2

NBD-F (4-Fluoro-7-nitrobenzofurazan) is a fluorescent derivatization agent used for amino acid analysis.

Pack Size	Price	USA Warehouse	Global Warehouse
25 mg	$38	In Stock	In Stock
50 mg	$53	In Stock	In Stock
100 mg	$86	In Stock	In Stock
1 mL x 10 mM (in DMSO)	$29	In Stock	In Stock

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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.

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Batch Information

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Purity:99.51%

Appearance:Solid

Color:White to Yellow

Resource Download

COA HNMR HPLC

Product Introduction

Bioactivity

Description	NBD-F (4-Fluoro-7-nitrobenzofurazan) is a fluorescent derivatization agent used for amino acid analysis.
In vitro	NBD-F for quantification of colistin sulfate in rat plasma 1. Sample preparation: Colistin sulfate (including CLA and CLB) was extracted from rat plasma using a solid phase extraction (SPE) C18 column. 2. Reaction with NBD-F: The extracted colistin sulfate was reacted with 2 mM NBD-F and 10 mM boric acid buffer (pH 9.5). 3. Heating reaction: The reaction mixture was heated at 60°C for 5 minutes to promote the derivatization reaction. 4. HPLC analysis: The derivatization products were analyzed using reversed-phase high performance liquid chromatography (HPLC) combined with fluorescence detection. 5. Quantitative analysis: The peaks of CLA and CLB were quantified using appropriate standards. The lower limit of quantification for CLA and CLB was 0.05 μg/mL.
Synonyms	4-Fluoro-7-nitrobenzofurazan

Chemical Properties

Molecular Weight	183.1
Formula	C₆H₂FN₃O₃
Cas No.	29270-56-2
Smiles	[O-][N+](=O)C1=CC=C(F)C2=NON=C12
Relative Density.	1.682g/cm3

Storage & Solubility Information

Storage

keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 110 mg/mL (600.76 mM), Sonication is recommended.

In Vivo Formulation

10% DMSO+40% PEG300+5% Tween-80+45% Saline: 3.3 mg/mL (18.02 mM), Sonication is recommended.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	5.4615 mL	27.3075 mL	54.6150 mL	273.0748 mL
5 mM	1.0923 mL	5.4615 mL	10.9230 mL	54.6150 mL
10 mM	0.5461 mL	2.7307 mL	5.4615 mL	27.3075 mL
20 mM	0.2731 mL	1.3654 mL	2.7307 mL	13.6537 mL
50 mM	0.1092 mL	0.5461 mL	1.0923 mL	5.4615 mL
100 mM	0.0546 mL	0.2731 mL	0.5461 mL	2.7307 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc