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NBD-F

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Catalog No. T19031Cas No. 29270-56-2
Alias 4-Fluoro-7-nitrobenzofurazan

NBD-F (4-Fluoro-7-nitrobenzofurazan) is a fluorescent derivatization agent used for amino acid analysis.

NBD-F

NBD-F

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Catalog No. T19031Alias 4-Fluoro-7-nitrobenzofurazanCas No. 29270-56-2
NBD-F (4-Fluoro-7-nitrobenzofurazan) is a fluorescent derivatization agent used for amino acid analysis.
Pack SizePriceAvailabilityQuantity
25 mg$38Backorder
50 mg$53Backorder
100 mg$86Backorder
1 mL x 10 mM (in DMSO)$29Backorder
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Product Introduction

Bioactivity
Description
NBD-F (4-Fluoro-7-nitrobenzofurazan) is a fluorescent derivatization agent used for amino acid analysis.
In vitro
NBD-F for quantification of colistin sulfate in rat plasma
1. Sample preparation: Colistin sulfate (including CLA and CLB) was extracted from rat plasma using a solid phase extraction (SPE) C18 column.
2. Reaction with NBD-F: The extracted colistin sulfate was reacted with 2 mM NBD-F and 10 mM boric acid buffer (pH 9.5).
3. Heating reaction: The reaction mixture was heated at 60°C for 5 minutes to promote the derivatization reaction.
4. HPLC analysis: The derivatization products were analyzed using reversed-phase high performance liquid chromatography (HPLC) combined with fluorescence detection.
5. Quantitative analysis: The peaks of CLA and CLB were quantified using appropriate standards. The lower limit of quantification for CLA and CLB was 0.05 μg/mL.
Alias4-Fluoro-7-nitrobenzofurazan
Chemical Properties
Molecular Weight183.1
FormulaC6H2FN3O3
Cas No.29270-56-2
Smiles[O-][N+](=O)C1=CC=C(F)C2=NON=C12
Relative Density.1.682g/cm3
Storage & Solubility Information
Storagekeep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice./Shipping at ambient temperature.
Solubility Information
DMSO: 110 mg/mL (600.76 mM), Sonication is recommended.
Solution Preparation Table
DMSO
1mg5mg10mg50mg
1 mM5.4615 mL27.3075 mL54.6150 mL273.0748 mL
5 mM1.0923 mL5.4615 mL10.9230 mL54.6150 mL
10 mM0.5461 mL2.7307 mL5.4615 mL27.3075 mL
20 mM0.2731 mL1.3654 mL2.7307 mL13.6537 mL
50 mM0.1092 mL0.5461 mL1.0923 mL5.4615 mL
100 mM0.0546 mL0.2731 mL0.5461 mL2.7307 mL

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