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Mutanolysin
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Catalog No. TRP-00260 Copy Product Info
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Mutanolysin belongs to the class of N-acetylmuramidases and is a biochemical reagent used for the lysis of Gram-positive bacteria. Mutanolysin effectively hydrolyzes the peptidoglycan in bacterial cell walls and is frequently utilized to prepare high-quality nucleic acids and intact ribosomal RNA from Gram-positive species like Listeria. It is widely employed in microbiology for cell lysis and molecular biology sample preparation.
Mutanolysin
Cas No. 55466-22-3
| Pack Size | Price | USA Stock | Global Stock | Quantity |
|---|---|---|---|---|
| 1 mg | $136 | - | In Stock |
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Appearance:Solid
Color:White to Yellow
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Product Introduction
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Chemical Properties
Storage & Solubility Information
| Description | Mutanolysin belongs to the class of N-acetylmuramidases and is a biochemical reagent used for the lysis of Gram-positive bacteria. Mutanolysin effectively hydrolyzes the peptidoglycan in bacterial cell walls and is frequently utilized to prepare high-quality nucleic acids and intact ribosomal RNA from Gram-positive species like Listeria. It is widely employed in microbiology for cell lysis and molecular biology sample preparation. |
| In vitro | In vitro, the use of Mutanolysin for the lysis of Listeria and other Gram-positive bacteria has been demonstrated to be a rapid and efficient method. This process achieves complete cell disruption while maintaining the structural integrity of intracellular macromolecules, such as ribosomal RNA, facilitating subsequent molecular detection and sequence analysis [1]. |
| Cas No. | 55466-22-3 |
| Storage | Powder: -20°C for 3 years | In solvent: -80°C for 1 year Shipping with blue ice/Shipping at ambient temperature. |
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In Vivo Formulation Calculator (Clear solution)
Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:
For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, and a total of 10 animals are to be administered, using a formulation of 
10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH2O , the resulting working solution concentration would be 2 mg/mL.Stock Solution Preparation:
Dissolve 2 mg of the compound in 100 μL DMSO to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.
Preparation of the In Vivo Formulation:
1) Add 100 μL of the DMSO stock solution to 400 µL PEG300 and mix thoroughly until the solution becomes clear.
2) Add 50 µL Tween 80 and mix well until fully clarified.
3) Add 450 µL Saline,PBS or ddH2O and mix thoroughly until a homogeneous solution is obtained.
This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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Related Tags: Mutanolysin in vitro
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