LW1564 is an HIF-1α inhibitor with an IC50 of 1.2 µM in HepG2 cells. It suppresses mitochondrial respiration, reduces ATP production, and stimulates HIF-1α degradation, thereby inhibiting the proliferation of various cancer cells with a GI50 ranging from 0.4 to 4.6 μM. Additionally, LW1564 activates the AMPK signaling pathway and inhibits lipid synthesis. In HepG2 xenograft mouse models, LW1564 demonstrates antitumor activity.