Luteolin (Standard)

Luteolin (Standard) is a reference compound for analytical and biochemical research involving Luteolin. Luteolin (Standard) belongs to the flavonoid class and functions as a Nrf2 inhibitor and PDE inhibitor. Luteolin (Standard) is utilized in biomedical research focused on flavonoid signaling modulation, oxidative stress regulation, inflammatory pathway analysis, and enzyme inhibition profiling across tumor, infectious, and allergic disease models.

Methods
Human lung cancer NCI-H460 cells were treated with luteolin at various concentrations for different durations. Cell viability, cell cycle, apoptosis and the expression of related proteins were detected.
Results
1.Luteolin at 0–160 μM inhibited the viability of NCI-H460 cells in a concentration-dependent manner after 24-hour treatment .
2.Treatment with 20–80 μM luteolin for 24 hours induced S-phase cell cycle arrest .
3.Luteolin at 320–580 μM induced cell apoptosis following 48-hour incubation .
4.After 24-hour treatment with 20–80 μM luteolin, the Bax/Bcl-2 ratio was upregulated in a concentration-dependent manner. Only 80 μM luteolin downregulated Bad expression, and Sirt1 protein levels were decreased dose-dependently within this concentration range [1].

Methods：Male Wistar rats with non-alcoholic steatohepatitis induced by high-fat and high-sugar diet were intragastrically administered luteolin at doses of 10–100 mg/kg daily for 12 consecutive weeks. The antioxidant capacity and changes of inflammation-related pathways were evaluated.
Results：Luteolin exhibited antioxidant activity. It targeted and inhibited the pro-inflammatory pathways of IL-1 and IL-18, thereby effectively preventing non-alcoholic steatohepatitis in rats [2].

DMSO: 80 mg/mL (279.49 mM), Sonication is recommended.