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LFM-A13

Name: LFM-A13
Brand: TargetMol
SKU: T212732
Rating: 4.5 (1 reviews)

Catalog No. T212732 Copy Product Info

Purity: 99.50%

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LFM-A13 is a highly selective inhibitor of the non-receptor tyrosine kinase BTK (Bruton's tyrosine kinase) (IC50 = 2.5 μM). It irreversibly severs the downstream signaling network of the B-cell receptor (BCR) by targeted binding to the BTK kinase catalytic domain, exhibiting excellent anti-leukemic and immunomodulatory effects in models of acute/chronic lymphocytic leukemia and severe autoimmune diseases.

LFM-A13

Copy Product Info

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Catalog No. T212732

Cas No. 62004-35-7

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Batch Information

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Purity:99.50%

Appearance:Solid

Color:White

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COA HNMR LCMS

Product Introduction

Bioactivity

Description	LFM-A13 is a highly selective inhibitor of the non-receptor tyrosine kinase BTK (Bruton's tyrosine kinase) (IC50 = 2.5 μM). It irreversibly severs the downstream signaling network of the B-cell receptor (BCR) by targeted binding to the BTK kinase catalytic domain, exhibiting excellent anti-leukemic and immunomodulatory effects in models of acute/chronic lymphocytic leukemia and severe autoimmune diseases.
In vitro	LFM-A13 (100 uM) inhibits Epo-induced phosphorylation of JAK2, BTK, STAT5, and ERK1/2 [1][2].
In vivo	LFM-A13 (10-100 mg/kg) exerts dose-dependent antitumor effects in breast cancer mouse models [3][4].

Chemical Properties

Molecular Weight	360
Formula	C₁₁H₈Br₂N₂O₂
Cas No.	62004-35-7
Smiles	N#CC(C(=O)NC1=CC(Br)=CC=C1Br)=C(O)C

Storage & Solubility Information

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 80.00 mg/mL (222.22 mM), Sonication is recommmended.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	2.7778 mL	13.8889 mL	27.7778 mL	138.8889 mL
5 mM	0.5556 mL	2.7778 mL	5.5556 mL	27.7778 mL
10 mM	0.2778 mL	1.3889 mL	2.7778 mL	13.8889 mL
20 mM	0.1389 mL	0.6944 mL	1.3889 mL	6.9444 mL
50 mM	0.0556 mL	0.2778 mL	0.5556 mL	2.7778 mL
100 mM	0.0278 mL	0.1389 mL	0.2778 mL	1.3889 mL

Note : The dilution table applies only to solid products. For liquid products, please calculate the stock solution based on the stated concentration and/or density.

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc