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JBSNF-000028

Name: JBSNF-000028
Brand: TargetMol
SKU: T72947
Price: 350 USD
Availability: InStock
Rating: 5 (10 reviews)

(Synonyms: JBSNF-000028 free base) Copy Product Info

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Synonyms: JBSNF-000028 free base

Catalog No. T72947 Copy Product Info

Purity: 99.24%

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JBSNF-000028 belongs to small molecule inhibitors, serving as a nicotinamide N-methyltransferase (NNMT) inhibitor (IC50=33 nM (human), 190 nM (monkey), 210 nM (mouse)), with oral activity and cell permeability, for research on metabolic disorders, possessing insulin-sensitizing, glucose-lowering, and weight-reducing effects.

Pack Size	Price	USA Stock	Global Stock
1 mg	$350	In Stock	In Stock
5 mg	$970	In Stock	In Stock
10 mg	$1,200	In Stock	In Stock
25 mg	$1,590	In Stock	In Stock
50 mg	$1,980	In Stock	In Stock
100 mg	$2,500	In Stock	In Stock

For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)

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Custom-made molecule. TargetMol’s expert synthesis team delivers cost-effective solutions. Contact us for inquiries. Committed to serving you.

For research use only—not for human use. No sales to individuals. Use as intended only.

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Purity:99.24%

Appearance:Solid

Color:White

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COA HNMR LCMS

Product Introduction

Bioactivity

Description	JBSNF-000028 belongs to small molecule inhibitors, serving as a nicotinamide N-methyltransferase (NNMT) inhibitor (IC50=33 nM (human), 190 nM (monkey), 210 nM (mouse)), with oral activity and cell permeability, for research on metabolic disorders, possessing insulin-sensitizing, glucose-lowering, and weight-reducing effects.
Targets & IC50	NNMT (human):0.033 μM, NNMT (monkey):0.19 μM, U2OS cells:2.5 μM (EC50), NNMT (mouse):0.21 μM
In vitro	Methods: The inhibitory effect of JBSNF-000028 hydrochloride on NNMT activity was evaluated in U2OS cells (24 hours); cytotoxicity was detected in HepG2 cells (72 hours); and the binding mechanism and selectivity were studied through structural analysis and broad target screening. Results: JBSNF-000028 hydrochloride inhibited NNMT activity with an EC50 of 2.5 μM; showed no cytotoxicity to HepG2 cells (10-100 μM); bound through a hairpin structure located beneath the nicotinamide pocket by stacking with Tyr-204 and Leu-164; and exhibited no activity against metabolism and safety-related target panels. [1]
In vivo	Methods: In diet-induced obese (DIO) mice and NNMT knockout DIO mice, JBSNF-000028 hydrochloride (50 mg/kg; twice daily) was administered orally for 27 days and 4 weeks, respectively, to evaluate its effects on glucose and lipid metabolism. Results: JBSNF-000028 hydrochloride improved glucose and lipid metabolism in DIO mice and enhanced glucose tolerance in NNMT knockout DIO mice. [1]
Synonyms	JBSNF-000028 free base

Chemical Properties

Molecular Weight	187.24
Formula	C₁₁H₁₃N₃
Smiles	CN1Cc2cccc3c2N(CC3)C1=N

Storage & Solubility Information

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 8.8 mg/mL (47 mM), Sonication is recommended.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	5.3407 mL	26.7037 mL	53.4074 mL	267.0370 mL
5 mM	1.0681 mL	5.3407 mL	10.6815 mL	53.4074 mL
10 mM	0.5341 mL	2.6704 mL	5.3407 mL	26.7037 mL
20 mM	0.2670 mL	1.3352 mL	2.6704 mL	13.3518 mL

Note : The dilution table applies only to solid products. For liquid products, please calculate the stock solution based on the stated concentration and/or density.

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.