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IPTG (Isopropyl β-D-thiogalactoside) is an unmetabolizable galactose analogue. IPTG can cause the transcriptional process of the lactose operon, and thus can induce the expression of the corresponding proteins of the downstream genes of the lactose operon.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
500 mg | $46 | In Stock | |
1 g | $53 | In Stock | |
5 g | $64 | In Stock | |
10 g | $98 | In Stock | |
25 g | $136 | In Stock | |
1 mL x 10 mM (in DMSO) | $48 | In Stock |
Description | IPTG (Isopropyl β-D-thiogalactoside) is an unmetabolizable galactose analogue. IPTG can cause the transcriptional process of the lactose operon, and thus can induce the expression of the corresponding proteins of the downstream genes of the lactose operon. |
In vitro | At low concentration, IPTG enters cells through lactose permease, but at high concentrations (typically used for protein induction), IPTG can enter the cells independently of lactose permease. |
Kinase Assay | For RNA interference (RNAi), A549 cells are plated at 1,500 cells per well in 96-well plates, allowed to adhere for 24 hours, and transfected with 25 nM siRNA oligonucleotide using Dharmafect 4. Transfected cells are treated with the indicated concentrations of GNE-617 (0.1, 1 , 10 , 100 , and 1000 nM) for 72 hours and viability is evaluated with CellTiter-Glo. Lysates for detection of NAPRT1 protein are collected 72 hours after transfection of 1 million A549 cells in 10 cm dishes. For NAPRT1 re-expression, RERF-LC-MS cells are transfected with pCMV6-AC.NAPRT1 and empty vector pCMV6-AC using Amaxa Nucleofector technology and selected with Geneticin |
Synonyms | Isopropyl β-D-thiogalactoside |
Molecular Weight | 238.3 |
Formula | C9H18O5S |
Cas No. | 367-93-1 |
Smiles | S(C(C)C)[C@@H]1O[C@H](CO)[C@H](O)[C@H](O)[C@H]1O |
Relative Density. | 1.37 g/cm3 |
Storage | store under nitrogen | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | |||||||||||||||||||||||||||||||||||
Solubility Information | DMSO: 60 mg/mL (251.78 mM), Sonication is recommended. ![]() | |||||||||||||||||||||||||||||||||||
Solution Preparation Table | ||||||||||||||||||||||||||||||||||||
DMSO
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