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HOE 32020

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Catalog No. T15495Cas No. 23554-99-6

HOE 32020 is a Hoechst stain, a blue fluorescent dyes, used to stain DNA. Hoechst dyes are soluble in water and in organic solvents such as dimethyl formamide or dimethyl sulfoxide. Concentrations can be achieved of up to 10 mg/mL. Aqueous solutions are stable at 2-6°C for at least six months when protected from light. For long-term storage the solutions are instead frozen at ≤-20°C.

HOE 32020

HOE 32020

Copy Product Info
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Catalog No. T15495Cas No. 23554-99-6
HOE 32020 is a Hoechst stain, a blue fluorescent dyes, used to stain DNA. Hoechst dyes are soluble in water and in organic solvents such as dimethyl formamide or dimethyl sulfoxide. Concentrations can be achieved of up to 10 mg/mL. Aqueous solutions are stable at 2-6°C for at least six months when protected from light. For long-term storage the solutions are instead frozen at ≤-20°C.
Pack SizePriceUSA WarehouseGlobal WarehouseQuantity
2 mg$198-In Stock
5 mg$347-In Stock
10 mg$541-In Stock
1 mL x 10 mM (in DMSO)$382-In Stock
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.
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Product Introduction

Bioactivity
Description
HOE 32020 is a Hoechst stain, a blue fluorescent dyes, used to stain DNA. Hoechst dyes are soluble in water and in organic solvents such as dimethyl formamide or dimethyl sulfoxide. Concentrations can be achieved of up to 10 mg/mL. Aqueous solutions are stable at 2-6°C for at least six months when protected from light. For long-term storage the solutions are instead frozen at ≤-20°C.
Chemical Properties
Molecular Weight442.94
FormulaC25H23ClN6
Cas No.23554-99-6
SmilesCN1CCN(CC1)c1ccc2[nH]c(nc2c1)-c1ccc2[nH]c(nc2c1)-c1ccc(Cl)cc1
Relative Density.1.356 g/cm3 (Predicted)
Storage & Solubility Information
Storagekeep away from direct sunlight | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
Solubility Information
DMSO: 69 mg/mL (155.78 mM), Sonication is recommended.
Solution Preparation Table
DMSO
1mg5mg10mg50mg
1 mM2.2576 mL11.2882 mL22.5764 mL112.8821 mL
5 mM0.4515 mL2.2576 mL4.5153 mL22.5764 mL
10 mM0.2258 mL1.1288 mL2.2576 mL11.2882 mL
20 mM0.1129 mL0.5644 mL1.1288 mL5.6441 mL
50 mM0.0452 mL0.2258 mL0.4515 mL2.2576 mL
100 mM0.0226 mL0.1129 mL0.2258 mL1.1288 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:
TargetMol | Animal experiments For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent 10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH2O , the resulting working solution concentration would be 2 mg/mL.
Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSOTargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSOTargetMol | reagent stock solution to 400 μL PEG300TargetMol | reagent and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH2OTargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.
All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.
1 Enter information below:
mg/kg
g
μL
2 Enter the in vivo formulation:
% DMSO
%
% Tween 80
% Saline/PBS/ddH2O

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

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