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Guadecitabine sodium

Name: Guadecitabine sodium
Brand: TargetMol
SKU: T12790
Rating: 4.5 (1 reviews)

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Catalog No. T12790Cas No. 929904-85-8

Alias SGI-110 sodium, S-110 sodium

Guadecitabine sodium is a inhibitor of second-generation DNA methyltransferases (DNMT) .

Guadecitabine sodium

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Catalog No. T12790Alias SGI-110 sodium, S-110 sodiumCas No. 929904-85-8

Guadecitabine sodium is a inhibitor of second-generation DNA methyltransferases (DNMT) .

Pack Size	Price	USA Warehouse	Global Warehouse
25 mg	$1,670	6-8 weeks	6-8 weeks
50 mg	$2,180	6-8 weeks	6-8 weeks
100 mg	$2,800	6-8 weeks	6-8 weeks

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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.

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Product Introduction

Bioactivity

Description	Guadecitabine sodium is a inhibitor of second-generation DNA methyltransferases (DNMT) .
In vitro	Treatment of HCT116 colorectal carcinoma cells with Guadecitabine sodium (SGI-110 sodium) demonstrated a dose-dependent increase in p16 expression after 6 days. Additionally, there was a similar increase in p16 protein levels in both T24 and HCT116 cells when treated with Guadecitabine sodium or 5-aza-CdR for 3 days. This effect suggests Guadecitabine sodium's effectiveness in inhibiting DNA methylation and stimulating p16 expression at both mRNA and protein levels, akin to 5-aza-CdR. Further, Guadecitabine sodium inhibited DNA methylation in the 5′-region and induced the expression of the p16 gene in T24 and HCT116 cells at concentrations comparable to those of 5-aza-CdR. The induction of p16 expression by both agents was associated with demethylation at the 5′-end region of the gene in both cell lines. In terms of toxicity, Guadecitabine sodium was slightly less toxic than 5-aza-CdR at concentrations up to 1 μM, but displayed similar toxicity levels at 10 μM concentration[1].
In vivo	Guadecitabine sodium (10mg/kg) effectively reduces DNA methylation, retards tumor growth, and demonstrates a toxicity level comparable to 5-Aza-CdR. It successfully reactivates the expression of the heavily methylated p16 gene in parent EJ6 cells and reduces DNA methylation at the p16 promoter region in vivo. Notably, Guadecitabine sodium exhibits better tolerance compared to 5-Aza-CdR in vivo[2].
Synonyms	SGI-110 sodium, S-110 sodium

Chemical Properties

Molecular Weight	580.407
Formula	C₁₈H₂₄N₉NaO₁₀P
Cas No.	929904-85-8
Smiles	[Na].[H][C@@]1(C[C@H](O)[C@@H](COP(O)(=O)O[C@H]2C[C@@]([H])(O[C@@H]2CO)n2cnc(N)nc2=O)O1)n1cnc2c1[nH]c(N)nc2=OjcExt:v:0:0
Relative Density.	1.31g/cm3

Storage & Solubility Information

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 50 mg/mL (86.15 mM), Sonication is recommended.

H2O: Soluble

In Vivo Formulation

10% DMSO+40% PEG300+5% Tween-80+45% Saline: 2.5 mg/mL (4.31 mM), Sonication is recommended.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	1.7229 mL	8.6146 mL	17.2293 mL	86.1464 mL
5 mM	0.3446 mL	1.7229 mL	3.4459 mL	17.2293 mL
10 mM	0.1723 mL	0.8615 mL	1.7229 mL	8.6146 mL
20 mM	0.0861 mL	0.4307 mL	0.8615 mL	4.3073 mL
50 mM	0.0345 mL	0.1723 mL	0.3446 mL	1.7229 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc