Ferroptosisinducer-12 is a potent and selective inducer of ferroptosis. It demonstrates strong antiproliferative activity against HT1080 and OS-RC-2 cell lines, with IC50 values of 3 nM and 5 nM, respectively. This compound significantly inhibits GPX4 enzyme activity, induces intracellular ROS, and elevates Fe2+ levels within OS-RC-2 cells. Moreover, Ferroptosisinducer-12 markedly inhibits tumor growth in BALB/c nude mice bearing OS-RC-2 xenografts. It is applicable for cancer research.

Ferroptosis inducer-12 (Compound A6) exhibits potent antiproliferative activity against HT1080 and OS-RC-2 cells over 48 hours, with IC50 values of 3 nM and 5 nM, respectively. Additionally, at a concentration of 1 μM for 2 hours, it strongly inhibits the enzymatic activity of glutathione peroxidase 4 (GPX4) in OS-RC-2 cells. When applied at 0.5 μM for 4 hours, it induces intracellular ROS, increases intracellular Fe2+ levels, and promotes lipid peroxidation (LPO) accumulation in OS-RC-2 cells. At a concentration of 10 nM for 24 hours, it significantly inhibits cell migration and reduces the invasiveness of OS-RC-2 cells.

Compound A6, a ferroptosis inducer, administered via intraperitoneal injection at 25-50 mg/kg every three days for 27 days, significantly inhibits tumor growth in BALB/c nude mice bearing OS-RC-2 xenografts.