Ferroptosisinducer-10 is an inducer of ferroptosis, capable of inhibiting the growth of A549 cells with an IC50 value of 0.76 μM. It functions by depleting glutathione (GSH), increasing reactive oxygen species (ROS) and malondialdehyde (MDA), and downregulating glutathione peroxidase 4 (GPX4) expression. Additionally, Ferroptosisinducer-10 induces cell cycle arrest at the G2/M phase and inhibits cell migration. This compound is applicable in cancer research, such as in studies of non-small cell lung cancer.

Ferroptosis inducer-10 (Compound I-21) exhibits antiproliferative activity against A549, HT-1080, MCF-7, Panc-02, and PC-3 cells with IC 50 values of 0.76, 1.03, 4.86, 3.41, and 1.34 μM, respectively. It shows no significant toxicity towards human umbilical vein endothelial cells (IC 50 = 24.66 μM). At concentrations of 0.5-4 μM, it increases reactive oxygen species and malondialdehyde levels while decreasing glutathione (GSH) levels in A549 cells. Ferroptosis inducer-10 elevates Fe 2+ levels in these cells at 0.5-4 μM after 12 hours， and causes cell shrinkage and fragmentation. Additionally, it downregulates glutathione peroxidase 4 levels and induces G2/M phase arrest in A549 cells at 1-4 μM over 24 hours. Colony formation is reduced at 0.5-2 μM over 7 days, and cell migration is inhibited at 0.5-4 μM over 48 hours.