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FabCOUPER protease
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Catalog No. TRP-00258
FabCOUPER protease is a serine protease that is recombinantly expressed in E.coli and features a 6×His tag at the C-terminus. It specifically cleaves human IgG1 at a single site above the hinge, efficiently generating intact Fab and Fc fragments within two hours without the need for reducing conditions.
FabCOUPER protease
FabCOUPER protease
😃Good
Catalog No. TRP-00258
FabCOUPER protease is a serine protease that is recombinantly expressed in E.coli and features a 6×His tag at the C-terminus. It specifically cleaves human IgG1 at a single site above the hinge, efficiently generating intact Fab and Fc fragments within two hours without the need for reducing conditions.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 10 mg | Inquiry | Backorder | |
| 50 mg | Inquiry | Backorder |
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Product Introduction
Bioactivity
Chemical Properties
| Description | FabCOUPER protease is a serine protease that is recombinantly expressed in E.coli and features a 6×His tag at the C-terminus. It specifically cleaves human IgG1 at a single site above the hinge, efficiently generating intact Fab and Fc fragments within two hours without the need for reducing conditions. |
Storage & Solubility Information
| Storage | Powder: -20°C for 3 years | In solvent: -80°C for 1 year |
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In Vivo Formulation Calculator (Clear solution)
Please enter your animal experiment information in the following box and click Calculate to obtain the mother liquor preparation method and in vivo formula preparation method:
For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . A total of 10 animals were administered, and the formula you used is 5% 
DMSO+30% PEG300+5% Tween 80+60% Saline/PBS/ddH2O. So your working solution concentration is 2 mg/mL。Mother liquor preparation method: 2 mg of drug dissolved in 50 μL DMSO (mother liquor concentration of 40 mg/mL), if you need to configure a concentration that exceeds the solubility of the product, please contact us first.
(mother liquor concentration of 40 mg/mL), if you need to configure a concentration that exceeds the solubility of the product, please contact us first.Preparation method for in vivo formula: Take 50 μL DMSO main solution, add 300 μLPEG300 mix well and clarify, then add 50 more μL Tween 80, mix well and clarify, then add 600 more μLSaline/PBS/ddH2O mix well and clarify
main solution, add 300 μLPEG300 mix well and clarify, then add 50 more μL Tween 80, mix well and clarify, then add 600 more μLSaline/PBS/ddH2O mix well and clarifyFor Reference Only. Please develop an appropriate dissolution method based on your laboratory animals and route of administration.
Dose Conversion
You can also refer to dose conversion for different animals. More Dose Conversion
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Tech Support
Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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