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Extracellular Death Factor TFA
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Catalog No. T36523L
Alias Extracellular Death Factor TFA（960129-66-2 Free bse）, EDF TFA
Extracellular Death Factor TFA (EDF TFA) is a linear pentapeptide that is produced and released by communicating cells and activates cell death pathways in cellular subpopulations when sufficient concentrations are reached.EDF TFA is sensitive to extreme pH, high temperatures, and other stressful conditions. At a concentration of 2.5 ng/ml, EDF has been shown to promote mazeEF (toxin-antitoxin system within bacteria) mediated cell death, promote the endonuclease activity of MazF toxin, lead to degradation of bacterial mRNA, and significantly reduce population size in E. coli cultures.
Extracellular Death Factor TFA
Extracellular Death Factor TFA
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Purity: 98.77%
Catalog No. T36523LAlias Extracellular Death Factor TFA（960129-66-2 Free bse）, EDF TFA
Extracellular Death Factor TFA (EDF TFA) is a linear pentapeptide that is produced and released by communicating cells and activates cell death pathways in cellular subpopulations when sufficient concentrations are reached.EDF TFA is sensitive to extreme pH, high temperatures, and other stressful conditions. At a concentration of 2.5 ng/ml, EDF has been shown to promote mazeEF (toxin-antitoxin system within bacteria) mediated cell death, promote the endonuclease activity of MazF toxin, lead to degradation of bacterial mRNA, and significantly reduce population size in E. coli cultures.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 1 mg | $43 | - | In Stock | |
| 5 mg | $98 | - | In Stock | |
| 10 mg | $143 | - | In Stock | |
| 25 mg | $238 | - | In Stock |
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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Batch Information
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Purity:98.77%
Appearance:Solid
Color:White
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Product Introduction
Bioactivity
Chemical Properties
Storage & Solubility Information
| Description | Extracellular Death Factor TFA (EDF TFA) is a linear pentapeptide that is produced and released by communicating cells and activates cell death pathways in cellular subpopulations when sufficient concentrations are reached.EDF TFA is sensitive to extreme pH, high temperatures, and other stressful conditions. At a concentration of 2.5 ng/ml, EDF has been shown to promote mazeEF (toxin-antitoxin system within bacteria) mediated cell death, promote the endonuclease activity of MazF toxin, lead to degradation of bacterial mRNA, and significantly reduce population size in E. coli cultures. |
| In vitro | Tryptophan may be a key residue in the hydroxyl radical scavenging activity of Extracellular Death Factor TFA, which can protect Escherichia coli from killing by bactericidal antibiotics but not from DNA damage or killing by bacteriostatic antibiotics. [1] |
| Synonyms | Extracellular Death Factor TFA（960129-66-2 Free bse）, EDF TFA |
| Sequence | Asn-Asn-Trp-Asn-Asn |
| Sequence Short | NNWNN |
| Storage | store at low temperature | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature. |
| Solubility Information | DMSO: 100 mg/mL, Sonication is recommended.  |
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In Vivo Formulation Calculator (Clear solution)
Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:
For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, and a total of 10 animals are to be administered, using a formulation of 
10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH2O , the resulting working solution concentration would be 2 mg/mL.Stock Solution Preparation:
Dissolve 2 mg of the compound in 100 μL DMSO to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.
Preparation of the In Vivo Formulation:
1) Add 100 μL of the DMSO stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.
2) Add 50 μL Tween 80 and mix well until fully clarified.
3) Add 450 μL Saline,PBS or ddH2O and mix thoroughly until a homogeneous solution is obtained.
This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.
Dose Conversion
You can also refer to dose conversion for different animals. More Dose Conversion
Tech Support
Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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