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Erythrosine B

Name: Erythrosine B
Brand: TargetMol
SKU: T0305
Price: 33 USD
Availability: InStock
Rating: 5 (10 reviews)

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Catalog No. T0305Cas No. 16423-68-0

Alias Erythrosine I, Erythrosin extra bluish, Erythrosin B

Erythrosine B (Erythrosine I) , a tetraiodofluorescein, is served as a red coloring in some foods (cherries, fish), as a disclosure of dental plaque, and as a stain of some cell types. Its structural was similar to thyroxine.

Erythrosine B

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Purity: 98.87%

Catalog No. T0305Alias Erythrosine I, Erythrosin extra bluish, Erythrosin BCas No. 16423-68-0

Pack Size	Price	USA Warehouse	Global Warehouse
50 mg	$33	In Stock	In Stock
100 mg	$47	In Stock	In Stock
200 mg	$68	In Stock	In Stock
500 mg	$112	In Stock	In Stock
1 mL x 10 mM (in DMSO)	$50	In Stock	In Stock

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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.

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Batch Information

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Purity:98.87%

Appearance:Solid

Color:Red

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COA LCMS HNMR

Product Introduction

Erythrosine B AI Summary

Erythrosine B exhibits significant bioactivity as an enzyme inhibitor. It has demonstrated inhibitory effects on various enzymes, including native rhodanese, soluble pig heart malate dehydrogenase (MDH), Escherichia coli GroEL, and human HSP60/HSP10, with varying potency as indicated by IC50 values. Notably, it also inhibits ATPase activity of E. coli GroEL. In addition to these effects, the compound significantly impacts the refolding processes of denatured rhodanese and soluble pig heart MDH, which are mediated by the GroEL/GroES complex. These properties suggest that Erythrosine B has potential as an enzyme inhibitor targeting diverse proteins involved in critical biochemical pathways..

Note: Summary generated by AI. Data source: ChEMBL

Bioactivity

Description	Erythrosine B (Erythrosine I) , a tetraiodofluorescein, is served as a red coloring in some foods (cherries, fish), as a disclosure of dental plaque, and as a stain of some cell types. Its structural was similar to thyroxine.
Cell Research	Instructions I. Solution preparation 1. Mother solution preparation: Dissolve Erythrosine B in an appropriate solvent, such as anhydrous DMSO, ethanol or water, to prepare a stock solution of the required concentration. The recommended concentration of the stock solution is 10-100 mM for subsequent dilution. 2. Working solution preparation: Dilute the mother solution into the working solution to be used according to the experimental purpose. II. Operation steps Cell staining 1. Culture the cells to an appropriate density. 2. Wash the cells with PBS and remove the culture medium. 3. Add the diluted Erythrosine B solution to the cells and incubate at 37°C in the dark for 15-30 minutes. 4. After incubation, wash the cells 3 times with PBS to remove unbound dye. 5. Fluorescence detection: Use a fluorescence microscope or flow cytometer for detection. The excitation wavelength of Erythrosine B is about 530 nm and the emission wavelength is about 550 nm. Precautions: 1) Wear gloves during operation to avoid contact between skin or mucous membranes and reagents. 2) Avoid light exposure during incubation and storage to prevent fluorescence quenching. 3) After staining, fluorescence detection and analysis should be performed immediately. The above information is based on published literature. Experimental procedures should be appropriately modified to meet specific research demands.
Synonyms	Erythrosine I, Erythrosin extra bluish, Erythrosin B

Chemical Properties

Molecular Weight	879.86
Formula	C₂₀H₆I₄Na₂O₅
Cas No.	16423-68-0
Smiles	C1=CC=C2C(=C1)C(=O)OC23C4=CC(=C(C(=C4OC5=C(C(=C(C=C35)I)[O-])I)I)[O-])I.[Na+].[Na+]
Relative Density.	0.98 g/cm3

Storage & Solubility Information

Storage

keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 60 mg/mL (68.19 mM), Sonication is recommended.

In Vivo Formulation

10% DMSO+40% PEG300+5% Tween-80+45% Saline: 2.5 mg/mL (2.84 mM), Sonication is recommended.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	1.1365 mL	5.6827 mL	11.3654 mL	56.8272 mL
5 mM	0.2273 mL	1.1365 mL	2.2731 mL	11.3654 mL
10 mM	0.1137 mL	0.5683 mL	1.1365 mL	5.6827 mL
20 mM	0.0568 mL	0.2841 mL	0.5683 mL	2.8414 mL
50 mM	0.0227 mL	0.1137 mL	0.2273 mL	1.1365 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc