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DO34

Name: DO34
Brand: TargetMol
SKU: T11070L
Price: 52 USD
Availability: InStock
Rating: 5 (10 reviews)

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Catalog No. T11070LCas No. 1848233-58-8

DO34 is a selective and potent diacylglycerol lipase (DAGL-α/β) inhibitor that impairs fear extinction in mice, and can be used to study lipopolysaccharide inflammatory pain.

DO34

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Purity: 99.55%

Catalog No. T11070LCas No. 1848233-58-8

DO34 is a selective and potent diacylglycerol lipase (DAGL-α/β) inhibitor that impairs fear extinction in mice, and can be used to study lipopolysaccharide inflammatory pain.

Pack Size	Price	USA Warehouse	Global Warehouse
1 mg	$52	In Stock	In Stock
5 mg	$123	In Stock	In Stock
10 mg	$197	In Stock	In Stock
25 mg	$369	In Stock	In Stock
50 mg	$546	In Stock	In Stock
100 mg	$787	In Stock	In Stock
200 mg	$1,070	In Stock	In Stock
1 mL x 10 mM (in DMSO)	$143	In Stock	In Stock

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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

This molecule is a custom-made product. TargetMol has an excellent synthesis team with the experience and capability to provide you with cost-effective products.If you have any questions, please feel free to contact us. We are committed to serving you wholeheartedly.

All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.

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Batch Information

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Purity:99.55%

Appearance:Solid

Color:White

Resource Download

COA FNMR HNMR LCMS

Product Introduction

Bioactivity

Description	DO34 is a selective and potent diacylglycerol lipase (DAGL-α/β) inhibitor that impairs fear extinction in mice, and can be used to study lipopolysaccharide inflammatory pain.
Targets&IC50	DAGLβ:3-8 nM, DAGLα:6 nM
In vitro	DO34 is a potent, selective and centrally active DAGL inhibitor, the efficiency of DAGLα in converting SAG to 2-AG was determined by a real-time fluorescence-based natural substrate assay using membrane lysates from HEK293T cells expressing recombinant human DAGLα, DAGLα IC50 = 6 nM.DO34 is also a potent DAGLβ inhibitor, DAGLβ DO34 is also a potent DAGLβ inhibitor, DAGLβ IC50 = 3-8 nM. [1]
In vivo	DO34 reduces 2-AG levels in the brain in a dose- and time-dependent manner and prevents fasting-induced regurgitation in mice, which is typical of cannabinoid CB1 receptor-mediated behavior, and DO34 blocks tonic CB1 activation.DO34 blocks basal PF-EPSCs in AM251-increased MAGL-TKO mice. [2]

Chemical Properties

Molecular Weight	531.53
Formula	C₂₆H₂₈F₃N₅O₄
Cas No.	1848233-58-8
Smiles	O=C(OC(C)(C)C)N1CCN(C(=O)N2N=NC(=C2)C=3C=CC(OC(F)(F)F)=CC3)C(CC=4C=CC=CC4)C1
Relative Density.	1.32 g/cm3 (Predicted)

Storage & Solubility Information

Storage

keep away from moisture | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 80 mg/mL (150.51 mM), Sonication is recommended.

In Vivo Formulation

10% DMSO+40% PEG300+5% Tween 80+45% Saline: 10 mg/mL (18.81 mM), Sonication is recommended.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	1.8814 mL	9.4068 mL	18.8136 mL	94.0681 mL
5 mM	0.3763 mL	1.8814 mL	3.7627 mL	18.8136 mL
10 mM	0.1881 mL	0.9407 mL	1.8814 mL	9.4068 mL
20 mM	0.0941 mL	0.4703 mL	0.9407 mL	4.7034 mL
50 mM	0.0376 mL	0.1881 mL	0.3763 mL	1.8814 mL
100 mM	0.0188 mL	0.0941 mL	0.1881 mL	0.9407 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc