DNH28

Name: DNH28
Brand: TargetMol
SKU: T211558
Rating: 4.5 (1 reviews)

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Catalog No. T211558

DNH28 is a potent NF-κB and MAPK inhibitor with an IC50 value of 0.93 μM in HepG2 cells. It induces apoptosis by downregulating Bcl-2 and upregulating BAX and Cleaved-caspase-3 expression. DNH28 can arrest the cell cycle and inhibit cell migration, and is applicable for research in hepatocellular carcinoma (HCC).

DNH28

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Catalog No. T211558

Pack Size	Price	USA Warehouse	Global Warehouse	Quantity
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50 mg	Inquiry	Inquiry	Inquiry

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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

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Product Introduction

Bioactivity

Description

In vitro

DNH28 (Compound 28) exhibits low toxicity to normal THLE-3 cells (IC 50 = 18.16 μM). At concentrations of 0.46-1.84 μM over 24 hours, DNH28 inhibits HepG2 cell proliferation by inducing apoptosis and causes dose-dependent arrest at the G2/M phase. It also suppresses HepG2 cell migration in a dose-dependent manner, with complete inhibition observed at 1.84 μM. Furthermore, DNH28 at 1.84 μM effectively blocks TNF-α-induced nuclear translocation of p65. The compound exerts its anti-hepatocellular carcinoma effects by inhibiting the activation of NF-κB and MAPK signaling pathways.

Chemical Properties

Storage & Solubility Information

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc